URACIL-DNA GLYCOSYLASE
Ligand-induced domain movement details

Jmol view of liganded conformation 1

Domain Movement

domain 1 is binding domain(fixed in space) domain 2 is binding domain(fixed in space)

Sequence


           __________________________________________________ 
 1flz(A) : ANELTWHDVLAEEKQQPHFLNTLQTVASERQSGVTIYPPQKDVFNAFRFT 
         :                                                    
 1eui(A) : ****TWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDVFNAFRFT 
           __________________________________________________ 

           _________________________78__82___________________ 
 1flz(A) : ELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIP 
         :                                                    
 1eui(A) : ELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIP 
           _________________________78__82___________________ 

           ____________116__________________________________1 
 1flz(A) : GFTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISL 
         :                                                    
 1eui(A) : GFTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISL 
           ____________116__________________________________1 

           53________________________________________________ 
 1flz(A) : INQHREGVVFLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCN 
         :                                                    
 1eui(A) : INQHREGVVFLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCN 
           53________________________________________________ 

           ____________________________                       
 1flz(A) : HFVLANQWLEQHGETPIDWMPVLPAESE                       
         :                                                    
 1eui(A) : HFVLANQWLEQRGETPIDWMPVLPA***                       
           ____________________________                       

Details

  Property   Value
 Conformer 1 1flz(A)
 Conformer 2 1eui(A)
 Radius gyration for conformer 1 16.6 A
 Radius gyration for conformer 2 16.95 A
 EC Number 3.2.2.3  
Click here to see the DynDom results or the famliy
 Trigger ligands URA230
 Trigger ligands in which conformation Conformation  1
 Conformaton with trigger ligands is compact Yes
 Spanning ligands Yes
 Residues in enzyme contacting ligands ALA76  ASN123  ASP64  GLN63  HIS187  LEU75  PHE77  PRO65  TYR66 
 Residues in extended bending regions contacting ligands ALA76  LEU75  PHE77 
 Residues in extended bending regions making H-bonds or salt-bridges with ligands PHE77 
  Residues in extended bending regions with an H-bond between its main chain and ligands PHE77 
Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT