Aristolochene synthase
Ligand-induced domain movement details

Jmol view of liganded conformation 1

Domain Movement

domain 1 is binding domain(fixed in space) domain 2 is binding domain(fixed in space)

Sequence


           _________23_______________________________________ 
 2oa6(D) : SLEPPPSTFQPLCHPLVEEVSKEVDGYFLQHWNFPNEKARKKFVAAGFSR 
         :                                                    
 2e4o(B) : SLEPPPSTFQPLCHPLVEEVSKEVDGYFLQHWNFPNEKARKKFVAAGFSR 
           _________23_______________________________________ 

           __________________________________________________ 
 2oa6(D) : VTCLYFPKALDDRIHFACRLLTVLFLIDDLLEYMSFEEGSAYNEKLIPIS 
         :                                                    
 2e4o(B) : VTCLYFPKALDDRIHFACRLLTVLFLIDDLLEYMSFEEGSAYNEKLIPIS 
           __________________________________________________ 

           _____________________________________________160__ 
 2oa6(D) : RGDVLPDRSIPVEYIIYDLWESMRAHDREMADEILEPVFLFMRAQTDRTR 
         :                                                    
 2e4o(B) : RGDVLPDRSIPVEYIIYDLWESMRAHDREMADEILEPVFLFMRAQTDRTR 
           _____________________________________________160__ 

           _____170______________________195_________________ 
 2oa6(D) : ARPMGLGGYLEYRERDVGKELLAALMRFSMGLKLSPSELQRVREIDANCS 
         :                                                    
 2e4o(B) : ARPMGLGGYLEYRERDVGKELLAALMRFSMGLKLSPSELQRVREIDANCS 
           _____170______________________195_________________ 

           __________________________________________________ 
 2oa6(D) : KHLSVVNDIYSYEKELYTSKTAHSEGGILCTSVQILAQEADVTAEAAKRV 
         :                                                    
 2e4o(B) : KHLSVVNDIYSYEKELY----------iLCTSVQILAQEADVTAEAAKRV 
           __________________________________________________ 

           ______________________________________________311_ 
 2oa6(D) : LFVMCREWELRHQLLVARLSAEGLETPGLAAYVEGLEYQMSGNELWSQTT 
         :                                                    
 2e4o(B) : LFVMCREWELRHQLLVARLSAEGLETPGLAAYVEGLEYQMSGNELWSQTT 
           ______________________________________________311_ 

           ______                                             
 2oa6(D) : LRYSVV                                             
         :                                                    
 2e4o(B) : LRYSV*                                             
           ______                                             

Details

  Property   Value
 Conformer 1 2oa6(D)
 Conformer 2 2e4o(B)
 Radius gyration for conformer 1 18.62 A
 Radius gyration for conformer 2 19.4 A
 EC Number 4.2.3.9  
Click here to see the DynDom results or the famliy
 Trigger ligands POP4293
 Trigger ligands in which conformation Conformation  1
 Conformaton with trigger ligands is compact Yes
 Spanning ligands Yes
 Residues in enzyme contacting ligands ARG175  ARG314  ASN219  ASP90  GLU227  LYS226  PHE87  SER223  TYR315 
 Residues in extended bending regions contacting ligands ARG314  TYR315 
 Residues in extended bending regions making H-bonds or salt-bridges with ligands ARG314  TYR315 
  Residues in extended bending regions with an H-bond between its main chain and ligands Null
Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT