ASPARTYL-TRNA SYNTHETASE
Ligand-induced domain movement details

Jmol view of liganded conformation 1

Domain Movement

domain 1 is binding domain(fixed in space) domain 2 is binding domain(fixed in space)

Sequence


           __________________________________________________ 
 1c0a(A) : MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGIVQVFFD 
         :                                                    
 1il2(A) : MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGIVQVFFD 
           __________________________________________________ 

           __________________________________________________ 
 1c0a(A) : PDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLT 
         :                                                    
 1il2(A) : PDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLT 
           __________________________________________________ 

           __________________________________________________ 
 1c0a(A) : IINRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRR 
         :                                                    
 1il2(A) : IINRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRR 
           __________________________________________________ 

           __________________________________________________ 
 1c0a(A) : FMDDHGFLDIETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQL 
         :                                                    
 1il2(A) : FMDDHGFLDIETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQL 
           __________________________________________________ 

           __________________________________________________ 
 1c0a(A) : LMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFMTAPQVREVMEA 
         :                                                    
 1il2(A) : LMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFMTAPQVREVMEA 
           __________________________________________________ 

           __________________271____278__283_287_____________ 
 1c0a(A) : LVRHLWLEVKGVDLGDFPVMTFAEAERRYGSDKPDLRNPMELTDVADLLK 
         :                                                    
 1il2(A) : LVRHLWLEVKGVDLGDFPVMTFAEAERRYGSDKPDLRNPMELTDVADLLK 
           __________________271____278__283_287_____________ 

           ____________________323___________________________ 
 1c0a(A) : SVEFAVFAGPANDPKGRVAALRVPGGASLTRKQIDEYGNFVKIYGAKGLA 
         :                                                    
 1il2(A) : SVEFAVFAGPANDPKGRVAALRVPGGASLTRKQIDEYGNFVKIYGAKGLA 
           ____________________323___________________________ 

           __________________________________________________ 
 1c0a(A) : YIKVNERAKGLEGINSPVAKFLNAEIIEDILDRTAAQDGDMIFFGADNKK 
         :                                                    
 1il2(A) : YIKVNERAKGLEGINSPVAKFLNAEIIEDILDRTAAQDGDMIFFGADNKK 
           __________________________________________________ 

           ____________415______424__________________________ 
 1c0a(A) : IVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP 
         :                                                    
 1il2(A) : IVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP 
           ____________415______424__________________________ 

           __________________________________________________ 
 1c0a(A) : FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVF 
         :                                                    
 1il2(A) : FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVF 
           __________________________________________________ 

           _____________________________________540__545_____ 
 1c0a(A) : GILGINEEEQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRD 
         :                                                    
 1il2(A) : GILGINEEEQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRD 
           _____________________________________540__545_____ 

           ___________________________________                
 1c0a(A) : VIAFPKTTAAACLMTEAPSFANPTALAELSIQVVK                
         :                                                    
 1il2(A) : VIAFPKTTAAACLMTEAPSFANPTALAELSIQVVK                
           ___________________________________                

Details

  Property   Value
 Conformer 1 1c0a(A)
 Conformer 2 1il2(A)
 Radius gyration for conformer 1 29.84 A
 Radius gyration for conformer 2 29.95 A
 EC Number 6.1.1.12  
Click here to see the DynDom results or the famliy
 Trigger ligands AMP800
 Trigger ligands in which conformation Conformation  1
 Conformaton with trigger ligands is compact Yes
 Spanning ligands No
 Residues in enzyme contacting ligands ALA350  ALA396  GLY348  LEU349  LYS347  PHE304  PRO367  VAL306  VAL368 
 Residues in extended bending regions contacting ligands Null
 Residues in extended bending regions making H-bonds or salt-bridges with ligands Null
  Residues in extended bending regions with an H-bond between its main chain and ligands Null
Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT