LYSINE-SENSITIVE ASPARTOKINASE 3
Ligand-induced domain movement details

Jmol view of liganded conformation 1

Domain Movement

domain 1 is binding domain(fixed in space) domain 2 is binding domain(fixed in space)

Sequence


           __________________________________________________ 
 2j0w(A) : EIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGITNLLVALA 
         :                                                    
 2j0x(A) : EIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGITNLLVALA 
           __________________________________________________ 

           __________________________________________________ 
 2j0w(A) : EGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA 
         :                                                    
 2j0x(A) : EGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA 
           __________________________________________________ 

           __________________________________________________ 
 2j0w(A) : ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRF 
         :                                                    
 2j0x(A) : ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRF 
           __________________________________________________ 

           __________________________________________________ 
 2j0w(A) : GRAEPDIAALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSD 
         :                                                    
 2j0x(A) : GRAEPDIAALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSD 
           __________________________________________________ 

           ___________________224_______________242_______252 
 2j0w(A) : YTAALLAEALHASRVDIWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMA 
         :                                                    
 2j0x(A) : YTAALLAEALHASRVDIWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMA 
           ___________________224_______________242_______252 

           __________________________________289_____________ 
 2j0w(A) : TFGAKVLHPATLLPAVRSDIPVFVGSSKDPRAGGTLVCNKTENPPLFRAL 
         :                                                    
 2j0x(A) : TFGAKVLHPATLLPAVRSDIPVFVGSSKDPRAGGTLVCNKTENPPLFRAL 
           __________________________________289_____________ 

           __________________________________________________ 
 2j0w(A) : ALRRNQTLLTLHSLNMLHSRGFLAEVFGILARHNISVDLITTSEVSVALT 
         :                                                    
 2j0x(A) : ALRRNQTLLTLHSLNMLHSRGFLAEVFGILARHNISVDLITTSEVSVALT 
           __________________________________________________ 

           __________________________________________________ 
 2j0w(A) : LDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALIGNDLSKACGV 
         :                                                    
 2j0x(A) : LDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALIGNDLSKACGV 
           __________________________________________________ 

           _______________________________________________    
 2j0w(A) : GKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE    
         :                                                    
 2j0x(A) : GKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE    
           _______________________________________________    

Details

  Property   Value
 Conformer 1 2j0w(A)
 Conformer 2 2j0x(A)
 Radius gyration for conformer 1 23.41 A
 Radius gyration for conformer 2 24.56 A
 EC Number 2.7.2.4  
Click here to see the DynDom results or the famliy
 Trigger ligands ADP820
 Trigger ligands in which conformation Conformation  1
 Conformaton with trigger ligands is compact Yes
 Spanning ligands Yes
 Residues in enzyme contacting ligands ALA256  ARG232  ASP222  ASP230  GLY10  GLY11  GLY225  ILE226  LYS257  LYS8  MET251  PRO231  THR221  THR229  TRP220  TYR227  VAL223  VAL258 
 Residues in extended bending regions contacting ligands ALA256  ASP222  GLY225  ILE226  LYS257  MET251  THR221  THR229  TYR227  VAL223  VAL258 
 Residues in extended bending regions making H-bonds or salt-bridges with ligands ASP222  LYS257  THR221  TYR227 
  Residues in extended bending regions with an H-bond between its main chain and ligands TYR227 
Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT