Isocitrate dehydrogenase [NADP] cytoplasmic
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space) domain 2 is binding domain(fixed in space)

Sequence


           __________________________________________________ 
 1t09(A) : MSKKISGGSVVEMQGDEMTRIIWELIKEKLIFPYVELDLHSYDLGIENRD 
         :                                                    
 1t0l(D) : MSKKISGGSVVEMQGDEMTRIIWELIKEKLIFPYVELDLHSYDLGIENRD 
           __________________________________________________ 

           __________________________________________________ 
 1t09(A) : ATNDQVTKDAAEAIKKHNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIR 
         :                                                    
 1t0l(D) : ATNDQVTKDAAEAIKKHNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIR 
           __________________________________________________ 

           _______________118____________133_________________ 
 1t09(A) : NILGGTVFREAIICKNIPRLVSGWVKPIIIGRHAYGDQYRATDFVVPGPG 
         :                                                    
 1t0l(D) : NILGGTVFREAIICKNIPRLVSGWVKPIIIGRHAYGDQYRATDFVVPGPG 
           _______________118____________133_________________ 

           __________________________________________________ 
 1t09(A) : KVEITYTPSDGTQKVTYLVHNFEEGGGVAMGMYNQDKSIEDFAHSSFQMA 
         :                                                    
 1t0l(D) : KVEITYTPSDGTQKVTYLVHNFEEGGGVAMGMYNQDKSIEDFAHSSFQMA 
           __________________________________________________ 

           __________________________________________________ 
 1t09(A) : LSKGWPLYLSTKNTILKKYDGRFKDIFQEIYDKQYKSQFEAQKIWYEHRL 
         :                                                    
 1t0l(D) : LSKGWPLYLSTKNTILKKYDGRFKDIFQEIYDKQYKSQFEAQKIWYEHRL 
           __________________________________________________ 

           ________________269_______________________________ 
 1t09(A) : IDDMVAQAMKSEGGFIWACKNYDGDVQSDSVAQGYGSLGMMTSVLVCPDG 
         :                                                    
 1t0l(D) : IDDMVAQAMKSEGGFIWACKNYDGDVQSDSVAQGYGSLGMMTSVLVCPDG 
           ________________269_______________________________ 

           __________________________________________________ 
 1t09(A) : KTVEAEAAHGTVTRHYRMYQKGQETSTNPIASIFAWTRGLAHRAKLDNNK 
         :                                                    
 1t0l(D) : KTVEAEAAHGTVTRHYRMYQKGQETSTNPIASIFAWTRGLAHRAKLDNNK 
           __________________________________________________ 

           __________________________________________________ 
 1t09(A) : ELAFFANALEEVSIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDK 
         :                                                    
 1t0l(D) : ELAFFANALEEVSIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDK 
           __________________________________________________ 

           ______________                                     
 1t09(A) : LGENLKIKLAQAKL                                     
         :                                                    
 1t0l(D) : LGENLKIKLAQAKL                                     
           ______________                                     

Details

  Property   Value
 Conformer 1 1t09(A)
 Conformer 2 1t0l(D)
 Radius gyration for conformer 1 23.36 A
 Radius gyration for conformer 2 23 A
 EC Number 1.1.1.42  
Click here to see the DynDom results or the famliy
 Trigger ligands ICT1619
 Trigger ligands in which conformation Conformation  2
 Conformaton with trigger ligands is compact Yes
 Spanning ligands Yes
 Residues in enzyme contacting ligands ALA308  ARG100  ARG109  ARG132  ASN96  ASP275  SER94  THR77  TYR139 
 Residues in extended bending regions contacting ligands ARG132  ASP275  TYR139 
 Residues in extended bending regions making H-bonds or salt-bridges with ligands ARG132  ASP275 
  Residues in extended bending regions with an H-bond between its main chain and ligands Null
Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT