S-adenosylmethionine synthetase isoform type-
Ligand-induced domain movement details

Jmol view of liganded conformation 1

Domain Movement

domain 1 is binding domain(fixed in space) domain 2 is binding domain(fixed in space)

Sequence


           __________________________________________________ 
 2obv(A) : FQSMGVFMFTSESVGEGHPDKICDQISDAVLDAHLKQDPNAKVACETVCK 
         :                                                    
 1qm4(A) : ****GAFMFTSESVGEGHPDKICDQISDAVLDAHLKQDPNAKVACETVCK 
           __________________________________________________ 

           ___________________________________________107____ 
 2obv(A) : TGMVLLCGEITSMAMVDYQRVVRDTIKHIGYDDSAKGFDFKTCNVLVALE 
         :                                                    
 1qm4(A) : TGMVLLCGEITSMAMIDYQRVVRDTIKHIGYDDSAKGFDFKTCNVLVALE 
           ___________________________________________108____ 

           _____________________________________151__________ 
 2obv(A) : QQSPDIAQCVHLDRNEEDVGAGDQGLMFGYATDETEECMPLTIILAHKLN 
         :                                                    
 1qm4(A) : QQSP------------eDVGAGDQGLMFGYATDETEECMPLTIVLAHKLN 
           _____________________________________152__________ 

           _______________________187_____195________________ 
 2obv(A) : ARMADLRRSGLLPWLRPDSKTQVTVQYMQDNGAVIPVRIHTIVISVQHNE 
         :                                                    
 1qm4(A) : TRMADLRRSGVLPWLRPDSKTQVTVQYVQDNGAVIPVRVHTIVISVQHNE 
           _______________________188_____196________________ 

           ______________________________________252_________ 
 2obv(A) : DITLEEMRRALKEQVIRAVVPAKYLDEDTVYHLQPSGRFVIGGPQGDAGV 
         :                                                    
 1qm4(A) : DITLEAMREALKEQVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGV 
           ______________________________________253_________ 

           __________________282_____________________________ 
 2obv(A) : TGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKAGLC 
         :                                                    
 1qm4(A) : TGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKAGLC 
           __________________283_____________________________ 

           _____________________________________________359__ 
 2obv(A) : RRVLVQVSYAIGVAEPLSISIFTYGTSQKTERELLDVVHKNFDLRPGVIV 
         :                                                    
 1qm4(A) : RRVLVQVSYAIGVAEPLSISIFTYGTSKKTERELLEVVNKNFDLRPGVIV 
           _____________________________________________360__ 

           __366________________385__________                 
 2obv(A) : RDLDLKKPIYQKTACYGHFGRSEFPWEVPRKLVF                 
         :                                                    
 1qm4(A) : RDLDLKKPIYQKTACYGHFGRSEFPWEVPKKLVF                 
           __367________________386__________                 

Details

  Property   Value
 Conformer 1 2obv(A)
 Conformer 2 1qm4(A)
 Radius gyration for conformer 1 21.15 A
 Radius gyration for conformer 2 21.26 A
 EC Number 2.5.1.6  
Click here to see the DynDom results or the famliy
 Trigger ligands SAM501
 Trigger ligands in which conformation Conformation  1
 Conformaton with trigger ligands is compact Yes
 Spanning ligands Yes
 Residues in enzyme contacting ligands ARG249  ASP179  ASP258  HIS29  ILE252  LYS181  PHE250  PRO30  SER206  SER247 
 Residues in extended bending regions contacting ligands ARG249  ASP258  ILE252  PHE250 
 Residues in extended bending regions making H-bonds or salt-bridges with ligands Null
  Residues in extended bending regions with an H-bond between its main chain and ligands Null
Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT