ALPHA-TREHALOSE-PHOSPHATE SYNTHASE
Ligand-induced domain movement details

Jmol view of liganded conformation 1

Domain Movement

domain 1 is binding domain(fixed in space) domain 2 is binding domain(fixed in space)

Sequence


           _____6_____________________________37_____________ 
 1gz5(C) : SRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSGETGNEDQ 
         :                                                    
 1uqt(B) : SRLVVVSNRIAPP------aGGLAVGILGALKAAGGLWFGWSGETGNEDQ 
           _____6_____________________________37_____________ 

           _53______61_______________________________________ 
 1gz5(C) : PLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRP 
         :                                                    
 1uqt(B) : PLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRP 
           _53______61_______________________________________ 

           __________________________________________________ 
 1gz5(C) : AWDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIG 
         :                                                    
 1uqt(B) : AWDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIG 
           __________________________________________________ 

           __________________________________________________ 
 1gz5(C) : FFLHIPFPTPEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNL 
         :                                                    
 1uqt(B) : FFLHIPFPTPEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNL 
           __________________________________________________ 

           __________________________________________________ 
 1gz5(C) : TRVTTRSAKSHTAWGKAFRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAE 
         :                                                    
 1uqt(B) : TRVTTRSAKSHTAWGKAFRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAE 
           __________________________________________________ 

           __________________________________________________ 
 1gz5(C) : LKNVQNIFSVERLDYSKGLPERFLAYEALLEKYPQHHGKIRYTQIAPTSR 
         :                                                    
 1uqt(B) : LKNVQNIFSVERLDYSKGLPERFLAYEALLEKYPQHHGKIRYTQIAPTSR 
           __________________________________________________ 

           __________________________________________________ 
 1gz5(C) : GDVQAYQDIRHQLENEAGRINGKYGQLGWTPLYYLNQHFDRKLL-kIFRY 
         :                                                    
 1uqt(B) : GDVQAYQDIRHQLENEAGRINGKYGQLGWTPLYYLNQHFDRKLLMKIFRY 
           __________________________________________________ 

           __________________________________________________ 
 1gz5(C) : SDVGLVTPLRDG-nLVAKEYVAAQDPANPGVLVLSQFAGAANELTSALIV 
         :                                                    
 1uqt(B) : SDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAANELTSALIV 
           __________________________________________________ 

           __________________________________________________ 
 1gz5(C) : NPYDRDEVAAALDRALT-sLAERISRHAE-lDVIVKNDINHWQECFISDL 
         :                                                    
 1uqt(B) : NPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISDL 
           __________________________________________________ 

           ______                                             
 1gz5(C) : KQIVPR                                             
         :                                                    
 1uqt(B) : KQIVPR                                             
           ______                                             

Details

  Property   Value
 Conformer 1 1gz5(C)
 Conformer 2 1uqt(B)
 Radius gyration for conformer 1 22.46 A
 Radius gyration for conformer 2 22.78 A
 EC Number 2.4.1.15  
Click here to see the DynDom results or the famliy
 Trigger ligands G6P901
UDP900
 Trigger ligands in which conformation Conformation  1
 Conformaton with trigger ligands is compact Yes
 Spanning ligands Yes
 Residues in enzyme contacting ligands ALA20  ARG262  ARG300  ARG341  ARG9  ASN364  ASP130  GLU369  GLY21  GLY22  HIS132  HIS154  HIS338  LEU23  LEU344  LEU365  LYS267  PHE339  PRO297  TRP85  TYR131  TYR76  VAL25  VAL260  VAL366 
 Residues in extended bending regions contacting ligands ALA20  ARG9  GLY21  GLY22  LEU23 
 Residues in extended bending regions making H-bonds or salt-bridges with ligands ARG9  GLY21  GLY22 
  Residues in extended bending regions with an H-bond between its main chain and ligands GLY21  GLY22 
Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT