NICOTINAMIDE MONONUCLEOTIDE ADENYLYL TRANSFER
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space) domain 2 is binding domain(fixed in space)

Sequence


           __________________________________________________ 
 1kku(A) : EKTEVVLLACGSFNPITNMHLRLFELAKDYMNGTGRYTVVKGIISPVGDA 
         :                                                    
 1kqo(E) : EKTEVVLLACGSFNPITNMHLRLFELAKDYMNGTGRYTVVKGIISPVGDA 
           __________________________________________________ 

           __________________________________________________ 
 1kku(A) : YKKKGLIPAYHRVIMAELATKNSKWVEVDTWESLQKEWKETLKVLRHHQE 
         :                                                    
 1kqo(E) : YKKKGLIPAYHRVIMAELATKNSKWVEVDTWESLQKEWKETLKVLRHHQE 
           __________________________________________________ 

           ______________158_________________178_____________ 
 1kku(A) : KLEASDvPKVKLLCGADLLESFAVPNLWKSEDITQIVANYGLICVTRAGN 
         :                                                    
 1kqo(E) : KLEAa-VPKVKLLCGADLLESFAVPNLWKSEDITQIVANYGLICVTRAGN 
           ______________158_________________178_____________ 

           __________________________________________________ 
 1kku(A) : DAQKFIYESDVLWKHRSNIHVVNEWFANDISSTKIRRALRRGQSIRYLVP 
         :                                                    
 1kqo(E) : DAQKFIYESDVLWKHRSNIHVVNEWIANDISSTKIRRALRRGQSIRYLVP 
           __________________________________________________ 

           __________________________________                 
 1kku(A) : DLVQEYIEKHNLYSSE******************                 
         :                                                    
 1kqo(E) : DLVQEYIEKHNLYSSESEDRNAGVILAPLQRNTA                 
           __________________________________                 

Details

  Property   Value
 Conformer 1 1kku(A)
 Conformer 2 1kqo(E)
 Radius gyration for conformer 1 17.65 A
 Radius gyration for conformer 2 17.38 A
 EC Number 2.7.7.1  
Click here to see the DynDom results or the famliy
 Trigger ligands DND486
 Trigger ligands in which conformation Conformation  2
 Conformaton with trigger ligands is compact Yes
 Spanning ligands Yes
 Residues in enzyme contacting ligands ASP158  ASP220  CYS14  CYS155  GLU215  GLU94  GLY15  GLY156  HIS24  LEU159  LEU168  LEU27  LYS57  MET23  PHE17  SER16  THR95  TRP169  TRP92  TYR55  VAL186  VAL51 
 Residues in extended bending regions contacting ligands ASP158  CYS155  GLY156  LEU159 
 Residues in extended bending regions making H-bonds or salt-bridges with ligands Null
  Residues in extended bending regions with an H-bond between its main chain and ligands Null
Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT