a2,3-sialyltransferase, a2,6-sialyltransferas
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

__________________________________________________   1rzu(B) : MNVLSVSSEIYPLIKTGGLADVVGALPIALEAHGVRTRTLIPGYPAVKAA           :                                                      1rzu(A) : MNVLSVSSEIYPLIKTGGLADVVGALPIALEAHGVRTRTLIPGYPAVKAA             __________________________________________________             __________________________________________________   1rzu(B) : VTDPVKCFEFTDLLGEKADLLEVQHERLDLLILDAPAYYERSGGPYLGQT           :                                                      1rzu(A) : VTDPVKCFEFTDLLGEKADLLEVQHERLDLLILDAPAYYERSGGPYLGQT             __________________________________________________             __________________________________________________   1rzu(B) : GKDYPDNWKRFAALSLAAARIGAGVLPGWRPDMVHAHDWQAAMTPVYMRY           :                                                      1rzu(A) : GKDYPDNWKRFAALSLAAARIGAGVLPGWRPDMVHAHDWQAAMTPVYMRY             __________________________________________________             __________________________________________________   1rzu(B) : AETPEIPSLLTIHNIAFQGQFGANIFSKLALPAHAFGMEGIEYYNDVSFL           :                                                      1rzu(A) : AETPEIPSLLTIHNIAFQGQFGANIFSKLALPAHAFGMEGIEYYNDVSFL             __________________________________________________             ________________________________________243_______   1rzu(B) : KGGLQTATALSTVSPSYAEEILTAEFGMGLEGVIGSRAHVLHGIVNGIDA           :                                                      1rzu(A) : KGGLQTATALSTVSPSYAEEILTAEFGMGLEGVIGSRAHVLHGIVNGIDA             ________________________________________243_______             __________________________________________________   1rzu(B) : DVWNPATDHLIHDNYSAANLKNRALNKKAVAEHFRIDDDGSPLFCVISRL           :                                                      1rzu(A) : DVWNPATDHLIHDNYSAANLKNRALNKKAVAEHFRIDDDGSPLFCVISRL             __________________________________________________             __________________________________________________   1rzu(B) : TWQKGIDLMAEAVDEIVSLGGRLVVLGAGDVALEGALLAAASRHHGRVGV           :                                                      1rzu(A) : TWQKGIDLMAEAVDEIVSLGGRLVVLGAGDVALEGALLAAASRHHGRVGV             __________________________________________________             ____________________373______________________398__   1rzu(B) : AIGYNEPLSHLMQAGCDAIIIPSRFEPCGLTQLYALRYGCIPVVARTGGL           :                                                      1rzu(A) : AIGYNEPLSHLMQAGCDAIIIPSRFEPCGLTQLYALRYGCIPVVARTGGL             ____________________373______________________398__             __________________________________________________   1rzu(B) : ADTVIDANHAALASKAATGVQFSPVTLDGLKQAIRRTVRYYHDPKLWTQM           :                                                      1rzu(A) : ADTVIDANHAALASKAATGVQFSPVTLDGLKQAIRRTVRYYHDPKLWTQM             __________________________________________________             _____458____________________                         1rzu(B) : QKLGMKSDVSWEKSAGLYAALYSQLISK                                 :                                                      1rzu(A) : QKLGMKSDVSWEKSAGLYAALYSQLIS*                                   _____458____________________

Details

Property   Value  Conformer 1 2c83(A)  Conformer 2 2ex1(A)  Radius gyration for conformer 1 22.9 A  Radius gyration for conformer 2 21.81 A  EC Number 2.4.99.4   2.4.99.6   2.4.99.9   Click here to see the DynDom results or the famliy  Trigger ligands C5P1427  Trigger ligands in which conformation Conformation  2  Conformaton with trigger ligands is compact Yes  Spanning ligands Yes  Residues in enzyme contacting ligands GLU338  GLY266  GLY310  HIS311  ILE335  LEU357  LEU37  LYS309  PHE337  PRO312  SER336  SER355  SER356  SER36  THR265   Residues in extended bending regions contacting ligands GLU338  ILE335  PHE337  SER336   Residues in extended bending regions making H-bonds or salt-bridges with ligands GLU338  PHE337  SER336    Residues in extended bending regions with an H-bond between its main chain and ligands PHE337  Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT