Aristolochene synthase
Ligand-induced domain movement details

Jmol view of liganded conformation 1

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

_________23_______________________________________   2oa6(D) : SLEPPPSTFQPLCHPLVEEVSKEVDGYFLQHWNFPNEKARKKFVAAGFSR           :                                                      2e4o(B) : SLEPPPSTFQPLCHPLVEEVSKEVDGYFLQHWNFPNEKARKKFVAAGFSR             _________23_______________________________________             __________________________________________________   2oa6(D) : VTCLYFPKALDDRIHFACRLLTVLFLIDDLLEYMSFEEGSAYNEKLIPIS           :                                                      2e4o(B) : VTCLYFPKALDDRIHFACRLLTVLFLIDDLLEYMSFEEGSAYNEKLIPIS             __________________________________________________             _____________________________________________160__   2oa6(D) : RGDVLPDRSIPVEYIIYDLWESMRAHDREMADEILEPVFLFMRAQTDRTR           :                                                      2e4o(B) : RGDVLPDRSIPVEYIIYDLWESMRAHDREMADEILEPVFLFMRAQTDRTR             _____________________________________________160__             _____170______________________195_________________   2oa6(D) : ARPMGLGGYLEYRERDVGKELLAALMRFSMGLKLSPSELQRVREIDANCS           :                                                      2e4o(B) : ARPMGLGGYLEYRERDVGKELLAALMRFSMGLKLSPSELQRVREIDANCS             _____170______________________195_________________             __________________________________________________   2oa6(D) : KHLSVVNDIYSYEKELYTSKTAHSEGGILCTSVQILAQEADVTAEAAKRV           :                                                      2e4o(B) : KHLSVVNDIYSYEKELY----------iLCTSVQILAQEADVTAEAAKRV             __________________________________________________             ______________________________________________311_   2oa6(D) : LFVMCREWELRHQLLVARLSAEGLETPGLAAYVEGLEYQMSGNELWSQTT           :                                                      2e4o(B) : LFVMCREWELRHQLLVARLSAEGLETPGLAAYVEGLEYQMSGNELWSQTT             ______________________________________________311_             ______                                               2oa6(D) : LRYSVV                                                       :                                                      2e4o(B) : LRYSV*                                                         ______

Details

Property   Value  Conformer 1 2oa6(D)  Conformer 2 2e4o(B)  Radius gyration for conformer 1 18.62 A  Radius gyration for conformer 2 19.4 A  EC Number 4.2.3.9   Click here to see the DynDom results or the famliy  Trigger ligands POP4293  Trigger ligands in which conformation Conformation  1  Conformaton with trigger ligands is compact Yes  Spanning ligands Yes  Residues in enzyme contacting ligands ARG175  ARG314  ASN219  ASP90  GLU227  LYS226  PHE87  SER223  TYR315   Residues in extended bending regions contacting ligands ARG314  TYR315   Residues in extended bending regions making H-bonds or salt-bridges with ligands ARG314  TYR315    Residues in extended bending regions with an H-bond between its main chain and ligands Null Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT