ASPARTATE AMINOTRANSFERASE
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

_______________________________35_________________   1akb(A) : SSWWSHVEMGPPDPILGVTEAFKRDTNSKKMNLGVGAYRDDNGKPYVLNC           :                                                      7aat(B) : SSWWSHVEMGPPDPILGVTEAFKRDTNSKKMNLGVGAYRDDNGKPYVLNC             _______________________________35_________________             __________________________________________________   1akb(A) : VRKAEAMIAAKKMDKEYLPIAGLADFTRASAELALGENSEAFKSGRYVTV           :                                                      7aat(B) : VRKAEAMIAAKKMDKEYLPIAGLADFTRASAELALGENSEAFKSGRYVTV             __________________________________________________             __________________________________________________   1akb(A) : QGISGTGSLRVGANFLQRFFKFSRDVYLPKPSWGNHTPIFRDAGLQLQAY           :                                                      7aat(B) : QGISGTGSLRVGANFLQRFFKFSRDVYLPKPSWGNHTPIFRDAGLQLQAY             __________________________________________________             __________________________________________________   1akb(A) : RYYDPKTCSLDFTGAMEDISKIPEKSIILLHACAHNPTGVDPRQEQWKEL           :                                                      7aat(B) : RYYDPKTCSLDFTGAMEDISKIPEKSIILLHACAHNPTGVDPRQEQWKEL             __________________________________________________             __________________________________________________   1akb(A) : ASVVKKRNLLAYFDMAYQGFASGDINRDAWALRHFIEQGIDVVLSQSYAH           :                                                      7aat(B) : ASVVKKRNLLAYFDMAYQGFASGDINRDAWALRHFIEQGIDVVLSQSYAK             __________________________________________________             __________________________________________________   1akb(A) : NMGLYGERAGAFTVICRDAEEAKRVESQLKILIRPMYSNPPMNGARIASL           :                                                      7aat(B) : NMGLYGERAGAFTVICRDAEEAKRVESQLKILIRPMYSNPPMNGARIASL             __________________________________________________             ________________327___________________________357_   1akb(A) : ILNTPELRKEWLVEVKGMADRIISMRTQLVSNLKKEGSSHNWQHITDQIG           :                                                      7aat(B) : ILNTPELRKEWLVEVKGMADRIISMRTQLVSNLKKEGSSHNWQHITDQIG             ________________327___________________________357_             __________________________________________________   1akb(A) : MFCFTGLKPEQVERLTKEFSIYMTKDGRISVAGVASSNVGYLAHAIHQVT           :                                                      7aat(B) : MFCFTGLKPEQVERLTKEFSIYMTKDGRISVAGVASSNVGYLAHAIHQVT             __________________________________________________             _                                                    1akb(A) : K                                                            :                                                      7aat(B) : K                                                              _

Details

Property   Value  Conformer 1 1akb(A)  Conformer 2 7aat(B)  Radius gyration for conformer 1 21.93 A  Radius gyration for conformer 2 22.09 A  EC Number 2.6.1.1   Click here to see the DynDom results or the famliy  Trigger ligands PLP258  Trigger ligands in which conformation Conformation  2  Conformaton with trigger ligands is compact No  Spanning ligands No  Residues in enzyme contacting ligands ALA224  ALA257  ARG266  ASN194  ASP222  GLY108  LEU112  LYS258  SER107  SER255  THR109  TRP140  TYR225   Residues in extended bending regions contacting ligands Null  Residues in extended bending regions making H-bonds or salt-bridges with ligands Null   Residues in extended bending regions with an H-bond between its main chain and ligands Null Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT