Glucokinase
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

__________________________________________________   1q18(A) : TKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAVIRVYLEE           :                                                      1sz2(B) : TKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAVIRVYLEE             __________________________________________________             __________________________________________________   1q18(A) : HKVEVKDGCIAIACPITGDWVAtNHTWAFSIAEkKNLGFSHLEIINDFTA           :                                                      1sz2(B) : HKVEVKDGCIAIACPITGDWVAtNHTWAFSIAEkKNLGFSHLEIINDFTA             __________________________________________________             _108__________________________________146_________   1q18(A) : VSaIPlKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLP           :                                                      1sz2(B) : VSaIPlKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLP             _108__________________________________146_________             __________________________________________________   1q18(A) : GEGGHVDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKA           :                                                      1sz2(B) : GEGGHVDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKA             __________________________________________________             __________________________________________________   1q18(A) : DNRLPENLKPKDITERALADSCTDCRRALSLFCVIgRFGGNLALNLGTFG           :                                                      1sz2(B) : DNRLPENLKPKDITERALADSCTDCRRALSLFCVIgRFGGNLALNLGTFG             __________________________________________________             ________________________________________299_______   1q18(A) : GVFIAGGIVPRFLEFFKASGFRAAFEDKGRFKEYVHDIPVYLIVHDNPGL           :                                                      1sz2(B) : GVFIAGGIVPRFLEFFKASGFRAAFEDKGRFKEYVHDIPVYLIVHDNPGL             ________________________________________299_______             _______________                                      1q18(A) : LGSGAHLRQTLGHIL                                              :                                                      1sz2(B) : LGSGAHLRQTLGHIL                                                _______________

Details

Property   Value  Conformer 1 1q18(A)  Conformer 2 1sz2(B)  Radius gyration for conformer 1 21.27 A  Radius gyration for conformer 2 20.73 A  EC Number 2.7.1.2   Click here to see the DynDom results or the famliy  Trigger ligands BGC2001  Trigger ligands in which conformation Conformation  2  Conformaton with trigger ligands is compact Yes  Spanning ligands Yes  Residues in enzyme contacting ligands ALA64  ASN99  ASP100  CYS65  GLU157  GLU187  GLY138  GLY140  HIS160  LEU139  PHE101  PRO66   Residues in extended bending regions contacting ligands Null  Residues in extended bending regions making H-bonds or salt-bridges with ligands Null   Residues in extended bending regions with an H-bond between its main chain and ligands Null Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT