glutathione synthetase
Ligand-induced domain movement details

Jmol view of liganded conformation 1

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

__________________________________________________   1m0w(A) : *PSKDQLNELIQEVNQWAITNGLSMYPPKFEENPSNASVSPVTIYPTPIP           :                                                      1m0t(B) : PPSKDQLNELIQEVNQWAITNGLSMYPPKFEENPSNASVSPVTIYPTPIP             __________________________________________________             __________________________________________________   1m0w(A) : RKCFDEAVQIQPVFNELYARITQDMAQPDSYLHKTTEALALSDSEFTGKL           :                                                      1m0t(B) : RKCFDEAVQIQPVFNELYARITQDMAQPDSYL------------eFTGKL             __________________________________________________             __________________________________________________   1m0w(A) : WSLYLATLKSAQYKKQNFRLGIFRSDYLIDKKKGTEQIKQVEFNTVSVSF           :                                                      1m0t(B) : WSLYLATLKSAQYKKQNFRLGIFRSDYLIDKK---eQIKQVEFNTVSVSF             __________________________________________________             __________________________________________________   1m0w(A) : AGLSEKVDRLHSYLNRANKYDPKGPIYNDQNMVISDSGYLLSKALAKAVE           :                                                      1m0t(B) : AGLSEKVDRLHSYLNRANKYDPKGPIYNDQNMVISDSGYLLSKALAKAVE             __________________________________________________             __________________________________________________   1m0w(A) : SYKSQQs-----DPIVAFIVQRNERNVFDQKVLELNLLEKFGTKSVRLTF           :                                                      1m0t(B) : SYKSQQSSSTTSDPIVAFIVQRNERNVFDQKVLELNLLEKFGTKSVRLTF             __________________________________________________             __________________________________________________   1m0w(A) : DDVNDKLFIDDKTGKLFIRDTEQEIAVVYYRTGYTTTDYTSEKDWEARLF           :                                                      1m0t(B) : DDVNDKLFIDDKTGKLFIRDTEQEIAVVYYRTGYTTTDYTSEKDWEARLF             __________________________________________________             ______________________________________________353_   1m0w(A) : LEKSFAIKAPDLLTQLSGSKKIQQLLTDEGVLGKYISDAEKKSSLLKTFV           :                                                      1m0t(B) : LEKSFAIKAPDLLTQLSGSKKIQQLLTDEGVLGKYISDAEKKSSLLKTFV             _____________________________________________1353_             __________________________________________________   1m0w(A) : KIYPLDDTKLGREGKRLALSEPSKYVLKPQREGGGNNVYKENIPNFLKGI           :                                                      1m0t(B) : KIYPLDDTKLGREGKRLALSEPSKYVLKPQ-----nNVYKENIPNFLKGI             __________________________________________________             _________416______________________________________   1m0w(A) : EERHWDAYILMELIEPELNENNIILRDNKSYNEPIISELGIYGCVLFNDE           :                                                      1m0t(B) : EERHWDAYILMELIEPELNENNIILRDNKSYNEPIISELGIYGCVLFNDE             ________1416______________________________________             _____________________________________                1m0w(A) : QVLSNEFSGSLLRSKFNTSNEGGVAAGFGCLDSIILY                        :                                                      1m0t(B) : QVLSNEFSGSLLRSKF------------gCLDSIILY                          _____________________________________

Details

Property   Value  Conformer 1 1m0w(A)  Conformer 2 1m0t(B)  Radius gyration for conformer 1 22.74 A  Radius gyration for conformer 2 23.97 A  EC Number 6.3.2.3   Click here to see the DynDom results or the famliy  Trigger ligands ANP504  Trigger ligands in which conformation Conformation  1  Conformaton with trigger ligands is compact Yes  Spanning ligands Yes  Residues in enzyme contacting ligands ASN391  GLU146  GLU386  GLU416  GLU442  GLY387  ILE418  LEU132  LEU417  LYS324  LYS382  LYS469  MET415  TYR393  VAL145  VAL380   Residues in extended bending regions contacting ligands GLU416  ILE418  LEU417  MET415   Residues in extended bending regions making H-bonds or salt-bridges with ligands GLU416  ILE418    Residues in extended bending regions with an H-bond between its main chain and ligands GLU416  ILE418  Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT