GLUTAMATE DEHYDROGENASE
Ligand-induced domain movement details

Jmol view of liganded conformation 1

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

_______________17_________________________________   1hwx(C) : ADREDDPNFFKMVEGFFDRGASIVEDKLVEDLKTRQTQEQKRNRVRGILR           :                                                      1nr1(C) : *****DPNFFKMVEGFFDRGASIVEDKLVEDLRTRESEEQKRNRVRGILR             _______________21_________________________________             __________________________________________________   1hwx(C) : IIKPCNHVLSLSFPIRRDDGSWEVIEGYRAQHSHQRTPCKGGIRYSTDVS           :                                                      1nr1(C) : IIKPCNHVLSLSFPIRRDDGSWEVIEGYRAQHSQHRTPCKGGIRYSTDVS             __________________________________________________             __________________________________________________   1hwx(C) : VDEVKALASLMTYKCAVVDVPFGGAKAGVKINPKNYTDEDLEKITRRFTM           :                                                      1nr1(C) : VDEVKALASLMTYKCAVVDVPFGGAKAGVKINPKNYTDNELEKITRRFTM             __________________________________________________             __________________________________________________   1hwx(C) : ELAKKGFIGPGVDVPAPNMSTGEREMSWIADTYASTIGHYDINAHACVTG           :                                                      1nr1(C) : ELAKKGFIGPGIDVPAPDMSTGEREMSWIADTYASTIGHYDINAHACVTG             __________________________________________________             ____207___________________________________________   1hwx(C) : KPISQGGIHGRISATGRGVFHGIENFIENASYMSILGMTPGFGDKTFAVQ           :                                                      1nr1(C) : KPISQGGIHGRISATGRGVFHGIENFINEASYMSILGMTPGFGDKTFVVQ             ____211___________________________________________             __________________________________________________   1hwx(C) : GFGNVGLHSMRYLHRFGAKCVAVGESDGSIWNPDGIDPKELEDFKLQHGT           :                                                      1nr1(C) : GFGNVGLHSMRYLHRFGAKCIAVGESDGSIWNPDGIDPKELEDFKLQHGS             __________________________________________________             __________________________________________________   1hwx(C) : ILGFPKAKIYEGSILEVDCDILIPAASEKQLTKSNAPRVKAKIIAEGANG           :                                                      1nr1(C) : ILGFPKAKPYEGSILEADCDILIPAASEKQLTKSNAPRVKAKIIAEGANG             __________________________________________________             ___________________________380____________________   1hwx(C) : PTTPQADKIFLERNIMVIPDLYLNAGGVTVSYFQILKNLNHVSYGRLTFK           :                                                      1nr1(C) : PTTPEADKIFLERNIMVIPDLYLNAGGVTVSYFEWLKNLNHVSYGRLTFK             ___________________________384____________________             __________________________________________________   1hwx(C) : YERDSNYHLLMSVQESLERKFGKHGGTIPIVPTAEFQDRISGASEKDIVH           :                                                      1nr1(C) : YERDSNYHLLMSVQESLERKFGKHGGTIPIVPTAEFQDRISGASEKDIVH             __________________________________________________             453_________________________481___________________   1hwx(C) : SGLAYTMERSARQIMRTAMKYNLGLDLRTAAYVNAIEKVFRVYNEAGVTF           :                                                      1nr1(C) : SGLAYTMEASARQIMRTAMKYNLGLDLRTAAYVNAIEKVFKVYNEAGVTF             457_________________________485___________________             _                                                    1hwx(C) : T                                                            :                                                      1nr1(C) : T                                                              _

Details

Property   Value  Conformer 1 1hwx(C)  Conformer 2 1nr1(C)  Radius gyration for conformer 1 25.11 A  Radius gyration for conformer 2 25.22 A  EC Number 1.4.1.3   Click here to see the DynDom results or the famliy  Trigger ligands NAI20  Trigger ligands in which conformation Conformation  1  Conformaton with trigger ligands is compact Yes  Spanning ligands Yes  Residues in enzyme contacting ligands ALA325  ALA326  ALA348  ARG211  ARG94  ASN168  ASN254  ASN349  ASN374  GLN250  GLN330  GLU275  GLY251  GLY253  GLY274  GLY347  MET169  PHE252  SER170  SER276  SER327  THR215  VAL255   Residues in extended bending regions contacting ligands ARG211   Residues in extended bending regions making H-bonds or salt-bridges with ligands ARG211    Residues in extended bending regions with an H-bond between its main chain and ligands Null Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT