INOSINE TRIPHOSPHATE PYROPHOSPHATASE
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space) domain 2 is binding domain(fixed in space)

Sequence


           ___________11______________27__________39___44____ 
 2car(A) : GSMAASLVGKKIVFVTGNAKKLEEVVQILGDKFPCTLVAQKIDLPEYQGE 
         :                                                    
 2j4e(D) : *SMAASLVGKKIVFVTGNAKKLEEVVQILGDKFPCTLVAQKIDLPEYQGE 
           ___________11______________27__________39___44____ 

           __________________________________________________ 
 2car(A) : PDEISIQKCQEAVRQVQGPVLVEDTCLCFNALGGLPGPYIKWFLEKLKPE 
         :                                                    
 2j4e(D) : PDEISIQKCQEAVRQVQGPVLVEDTCLCFNALGGLPGPYIKWFLEKLKPE 
           __________________________________________________ 

           __________________________________________________ 
 2car(A) : GLHQLLAGFEDKSAYALCTFALSTGDPSQPVRLFRGRTSGRIVAPRGCQD 
         :                                                    
 2j4e(D) : GLHQLLAGFEDKSAYALCTFALSTGDPSQPVRLFRGRTSGRIVAPRGCQD 
           __________________________________________________ 

           ______________________________________________     
 2car(A) : FGWDPCFQPDGYEQTYAEMPKAEKNAVSHRFRALLELQEYFGSLAA     
         :                                                    
 2j4e(D) : FGWDPCFQPDGYEQTYAEMPKAEKNAVSHRFRALLELQEYFGSLAA     
           ______________________________________________     

Details

  Property   Value
 Conformer 1 2car(A)
 Conformer 2 2j4e(D)
 Radius gyration for conformer 1 17.35 A
 Radius gyration for conformer 2 16.89 A
 EC Number 3.6.1.19  
Click here to see the DynDom results or the famliy
 Trigger ligands POP1000
 Trigger ligands in which conformation Conformation  2
 Conformaton with trigger ligands is compact Yes
 Spanning ligands Yes
 Residues in enzyme contacting ligands ASN16  GLU44  GLU71  GLY15  LYS19  LYS56  LYS89  THR14 
 Residues in extended bending regions contacting ligands GLU44  GLY15  THR14 
 Residues in extended bending regions making H-bonds or salt-bridges with ligands GLU44  GLY15  THR14 
  Residues in extended bending regions with an H-bond between its main chain and ligands GLY15 
Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT