Isocitrate dehydrogenase [NADP]
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

__________________________________________________   1sjs    : ESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPAMLKVVDA           :                                                      1hj6(A) : *SKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPAMLKVVDA             __________________________________________________             __________________________________________________   1sjs    : AVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKG           :                                                      1hj6(A) : AVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKG             __________________________________________________             _____________________125_____133__________________   1sjs    : PLTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVI           :                                                      1hj6(A) : PLTTPVGGGIRELNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVI             _____________________125_____133__________________             __________________________________________________   1sjs    : FRENSEDIYAGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPC           :                                                      1hj6(A) : FRENSEDIYAGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPC             __________________________________________________             __________________________________________________   1sjs    : SEEGTKRLVRAAIEYAIANDRDSVTLVHKGNIMKFTEGAFKDWGYQLARE           :                                                      1hj6(A) : SEEGTKRLVRAAIEYAIANDRDSVTLVHKGNIMKFTEGAFKDWGYQLARE             __________________________________________________             __________________________________________________   1sjs    : EFGGELIDGGPWLKVKNPNTGKEIVIKDVIADAFLQQILLRPAEYDVIAC           :                                                      1hj6(A) : EFGGELIDGGPWLKVKNPNTGKEIVIKDVIADAFLQQILLRPAEYDVIAC             __________________________________________________             ________________320_______________________________   1sjs    : MNLNGDYISDALAAQVGGIGIAPGANIGDECALFEATHGTAPKYAGQDKV           :                                                      1hj6(A) : MNLNGDYISDALAAQVGGIGIAPGANIGDECALFEATHGTAPKYAGQDKV             ________________320_______________________________             __________________________________________________   1sjs    : NPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYDFERLMDGAK           :                                                      1hj6(A) : NPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYDFERLMDGAK             __________________________________________________             _______________                                      1sjs    : LLKCSEFGDAIIENM                                              :                                                      1hj6(A) : LLKCSEFGDAIIENM                                                _______________

Details

Property   Value  Conformer 1 1sjs( )  Conformer 2 1hj6(A)  Radius gyration for conformer 1 22.75 A  Radius gyration for conformer 2 22.49 A  EC Number 1.1.1.42   Click here to see the DynDom results or the famliy  Trigger ligands GOL1417 GOL1418 IPM1419 NAP1420  Trigger ligands in which conformation Conformation  2  Conformaton with trigger ligands is compact Yes  Spanning ligands Yes  Residues in enzyme contacting ligands ALA333  ALA342  ARG119  ARG129  ARG153  ARG96  ASN115  ASN352  ASP307  ASP330  ASP392  CYS332  GLU113  GLU336  GLY321  GLY329  GLY340  HIS339  HIS366  ILE320  ILE328  ILE37  LEU103  LEU16  LYS100  PHE152  PRO102  THR104  THR338  THR341  TYR160  TYR216  TYR345  TYR391  VAL116  VAL351   Residues in extended bending regions contacting ligands ARG129  ASP330  CYS332  GLY321  GLY329  ILE320  ILE328   Residues in extended bending regions making H-bonds or salt-bridges with ligands ARG129    Residues in extended bending regions with an H-bond between its main chain and ligands Null Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT