Isocitrate dehydrogenase [NADP] cytoplasmic
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

__________________________________________________   1t09(A) : MSKKISGGSVVEMQGDEMTRIIWELIKEKLIFPYVELDLHSYDLGIENRD           :                                                      1t0l(D) : MSKKISGGSVVEMQGDEMTRIIWELIKEKLIFPYVELDLHSYDLGIENRD             __________________________________________________             __________________________________________________   1t09(A) : ATNDQVTKDAAEAIKKHNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIR           :                                                      1t0l(D) : ATNDQVTKDAAEAIKKHNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIR             __________________________________________________             _______________118____________133_________________   1t09(A) : NILGGTVFREAIICKNIPRLVSGWVKPIIIGRHAYGDQYRATDFVVPGPG           :                                                      1t0l(D) : NILGGTVFREAIICKNIPRLVSGWVKPIIIGRHAYGDQYRATDFVVPGPG             _______________118____________133_________________             __________________________________________________   1t09(A) : KVEITYTPSDGTQKVTYLVHNFEEGGGVAMGMYNQDKSIEDFAHSSFQMA           :                                                      1t0l(D) : KVEITYTPSDGTQKVTYLVHNFEEGGGVAMGMYNQDKSIEDFAHSSFQMA             __________________________________________________             __________________________________________________   1t09(A) : LSKGWPLYLSTKNTILKKYDGRFKDIFQEIYDKQYKSQFEAQKIWYEHRL           :                                                      1t0l(D) : LSKGWPLYLSTKNTILKKYDGRFKDIFQEIYDKQYKSQFEAQKIWYEHRL             __________________________________________________             ________________269_______________________________   1t09(A) : IDDMVAQAMKSEGGFIWACKNYDGDVQSDSVAQGYGSLGMMTSVLVCPDG           :                                                      1t0l(D) : IDDMVAQAMKSEGGFIWACKNYDGDVQSDSVAQGYGSLGMMTSVLVCPDG             ________________269_______________________________             __________________________________________________   1t09(A) : KTVEAEAAHGTVTRHYRMYQKGQETSTNPIASIFAWTRGLAHRAKLDNNK           :                                                      1t0l(D) : KTVEAEAAHGTVTRHYRMYQKGQETSTNPIASIFAWTRGLAHRAKLDNNK             __________________________________________________             __________________________________________________   1t09(A) : ELAFFANALEEVSIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDK           :                                                      1t0l(D) : ELAFFANALEEVSIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDK             __________________________________________________             ______________                                       1t09(A) : LGENLKIKLAQAKL                                               :                                                      1t0l(D) : LGENLKIKLAQAKL                                                 ______________

Details

Property   Value  Conformer 1 1t09(A)  Conformer 2 1t0l(D)  Radius gyration for conformer 1 23.36 A  Radius gyration for conformer 2 23 A  EC Number 1.1.1.42   Click here to see the DynDom results or the famliy  Trigger ligands ICT1619  Trigger ligands in which conformation Conformation  2  Conformaton with trigger ligands is compact Yes  Spanning ligands Yes  Residues in enzyme contacting ligands ALA308  ARG100  ARG109  ARG132  ASN96  ASP275  SER94  THR77  TYR139   Residues in extended bending regions contacting ligands ARG132  ASP275  TYR139   Residues in extended bending regions making H-bonds or salt-bridges with ligands ARG132  ASP275    Residues in extended bending regions with an H-bond between its main chain and ligands Null Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT