LYSINE-SENSITIVE ASPARTOKINASE 3
Ligand-induced domain movement details

Jmol view of liganded conformation 1

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

__________________________________________________   2j0w(A) : EIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGITNLLVALA           :                                                      2j0x(A) : EIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGITNLLVALA             __________________________________________________             __________________________________________________   2j0w(A) : EGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA           :                                                      2j0x(A) : EGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA             __________________________________________________             __________________________________________________   2j0w(A) : ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRF           :                                                      2j0x(A) : ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRF             __________________________________________________             __________________________________________________   2j0w(A) : GRAEPDIAALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSD           :                                                      2j0x(A) : GRAEPDIAALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSD             __________________________________________________             ___________________224_______________242_______252   2j0w(A) : YTAALLAEALHASRVDIWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMA           :                                                      2j0x(A) : YTAALLAEALHASRVDIWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMA             ___________________224_______________242_______252             __________________________________289_____________   2j0w(A) : TFGAKVLHPATLLPAVRSDIPVFVGSSKDPRAGGTLVCNKTENPPLFRAL           :                                                      2j0x(A) : TFGAKVLHPATLLPAVRSDIPVFVGSSKDPRAGGTLVCNKTENPPLFRAL             __________________________________289_____________             __________________________________________________   2j0w(A) : ALRRNQTLLTLHSLNMLHSRGFLAEVFGILARHNISVDLITTSEVSVALT           :                                                      2j0x(A) : ALRRNQTLLTLHSLNMLHSRGFLAEVFGILARHNISVDLITTSEVSVALT             __________________________________________________             __________________________________________________   2j0w(A) : LDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALIGNDLSKACGV           :                                                      2j0x(A) : LDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALIGNDLSKACGV             __________________________________________________             _______________________________________________      2j0w(A) : GKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE              :                                                      2j0x(A) : GKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE                _______________________________________________

Details

Property   Value  Conformer 1 2j0w(A)  Conformer 2 2j0x(A)  Radius gyration for conformer 1 23.41 A  Radius gyration for conformer 2 24.56 A  EC Number 2.7.2.4   Click here to see the DynDom results or the famliy  Trigger ligands ADP820  Trigger ligands in which conformation Conformation  1  Conformaton with trigger ligands is compact Yes  Spanning ligands Yes  Residues in enzyme contacting ligands ALA256  ARG232  ASP222  ASP230  GLY10  GLY11  GLY225  ILE226  LYS257  LYS8  MET251  PRO231  THR221  THR229  TRP220  TYR227  VAL223  VAL258   Residues in extended bending regions contacting ligands ALA256  ASP222  GLY225  ILE226  LYS257  MET251  THR221  THR229  TYR227  VAL223  VAL258   Residues in extended bending regions making H-bonds or salt-bridges with ligands ASP222  LYS257  THR221  TYR227    Residues in extended bending regions with an H-bond between its main chain and ligands TYR227  Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT