MRNA CAPPING ENZYME
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

__________________________________________________   1cko    : NITTERAVLTLNGLQIKLHKVVGESRDDIVAKMKDLAMDDHKFPRLPGPN           :                                                      1ckm(B) : NITTERAVLTLNGLQIKLHKVVGESRDDIVAKMKDLAMDDHKFPRLPGPN             __________________________________________________             __________________________________________________   1cko    : PVSIERKDFEKLKQNKYVVSEKTDGIRFMMFFTRVFGFKVCTIIDRAMTV           :                                                      1ckm(B) : PVSIERKDFEKLKQNKYVVSEKTDGIRFMMFFTRVFGFKVCTIIDRAMTV             __________________________________________________             __________________________________________________   1cko    : YLLPFKNIPRVLFQGSIFDGELCVDIVEKKFAFVLFDAVVVSGVTVSQMD           :                                                      1ckm(B) : YLLPFKNIPRVLFQGSIFDGELCVDIVEKKFAFVLFDAVVVSGVTVSQMD             __________________________________________________             __________________________________________________   1cko    : LASRFFAMKRSLKEFKNVPEDPAILRYKEWIPLEHPTIIKDHLKKANAIY           :                                                      1ckm(B) : LASRFFAMKRSLKEFKNVPEDPAILRYKEWIPLEHPTIIKDHLKKANAIY             __________________________________________________             ______________________235_________________________   1cko    : HTDGLIIMSVDEPVIYGRNFNLFKLKPGTHHTIDFIIMSEDGTIGIFDPN           :                                                      1ckm(B) : HTDGLIIMSVDEPVIYGRNFNLFKLKPGTHHTIDFIIMSEDGTIGIFDPN             ______________________235_________________________             ______________________________________________309_   1cko    : LRKNVPVGKLDGYYNKGSIVECGFADGTWKYIQGRSDKNQANDRLTYEKT           :                                                      1ckm(B) : LRKNVPVGKLDGYYNKGSIVECGFADGTWKYIQGRSDKNQANDRLTYEKT             ______________________________________________309_             _________________                                    1cko    : LLNIEENITIDELLDLF                                            :                                                      1ckm(B) : LLNIEENITIDELLDLF                                              _________________

Details

Property   Value  Conformer 1 1cko( )  Conformer 2 1ckm(B)  Radius gyration for conformer 1 21.27 A  Radius gyration for conformer 2 19.9 A  EC Number 2.7.7.50   Click here to see the DynDom results or the famliy  Trigger ligands GTP999  Trigger ligands in which conformation Conformation  2  Conformaton with trigger ligands is compact Yes  Spanning ligands Yes  Residues in enzyme contacting ligands ARG106  ARG228  ARG295  ARG87  ASN302  ASP244  GLU131  ILE216  LEU232  LYS188  LYS234  LYS236  LYS298  LYS82  PHE146  PRO59  PRO61  THR83  TRP190   Residues in extended bending regions contacting ligands ASP244  LEU232  LYS234  LYS236   Residues in extended bending regions making H-bonds or salt-bridges with ligands ASP244  LYS234  LYS236    Residues in extended bending regions with an H-bond between its main chain and ligands Null Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT