Nitrogenase Molybdenum-Iron Protein alpha cha
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

__________________________________________________   1l5h(A) : *********************************************NKKSQ           :                                                      1m1n(E) : MSREEVESLIQEVLEVYPEKARKDRNKHLAVNDPAVTQSKKCIISNKKSQ             __________________________________________________             __________________________________________________   1l5h(A) : PGLMTIRGCAYAGSKGVVWGPIKDMIHISHGPVGCGQYSRAGRRNYYIGT           :                                                      1m1n(E) : PGLMTIRGCAYAGSKGVVWGPIKDMIHISHGPVGCGQYSRAGRRNYYIGT             __________________________________________________             __________________________________________________   1l5h(A) : TGVNAFVTMNFTSDFQEKDIVFGGDKKLAKLIDEVETLFPLNKGISVQSE           :                                                      1m1n(E) : TGVNAFVTMNFTSDFQEKDIVFGGDKKLAKLIDEVETLFPLNKGISVQSE             __________________________________________________             __________________________________________________   1l5h(A) : CPIGLIGDDIESVSKVKGAELSKTIVPVRCEGFRGVSQSLGHHIANDAVR           :                                                      1m1n(E) : CPIGLIGDDIESVSKVKGAELSKTIVPVRCEGFRGVSQSLGHHIANDAVR             __________________________________________________             __________________________________________________   1l5h(A) : DWVLGKRDEDTTFASTPYDVAIIGDYNIGGDAWSSRILLEEMGLRCVAQW           :                                                      1m1n(E) : DWVLGKRDEDTTFASTPYDVAIIGDYNIGGDAWSSRILLEEMGLRCVAQW             __________________________________________________             __________________________________________________   1l5h(A) : SGDGSISEIELTPKVKLNLVHCYRSMNYISRHMEEKYGIPWMEYNFFGPT           :                                                      1m1n(E) : SGDGSISEIELTPKVKLNLVHCYRSMNYISRHMEEKYGIPWMEYNFFGPT             __________________________________________________             ____________________________________________350___   1l5h(A) : KTIESLRAIAAKFDESIQKKCEEVIAKYKPEWEAVVAKYRPRLEGKRVML           :                                                      1m1n(E) : KTIESLRAIAAKFDESIQKKCEEVIAKYKPEWEAVVAKYRPRLEGKRVML             ____________________________________________350___             _357______________374_____________________________   1l5h(A) : YIGGLRPRHVIGAYEDLGMEVVGTGYE-----------------------           :                                                      1m1n(E) : YIGGLRPRHVIGAYEDLGMEVVGTGYEFAHNDDYDRTMKEMGDSTLLYDD             _357______________374_____________________________             _____411_____419____426___________________________   1l5h(A) : ----eFEEFVKRIKPDLIGSGIKEKFIFQKMGIPFREMHSWDYSGPYHGF           :                                                      1m1n(E) : VTGYEFEEFVKRIKPDLIGSGIKEKFIFQKMGIPFREMHSWDYSGPYHGF             _____411_____419____426___________________________             ___________________________                          1l5h(A) : DGFAIFARDMDMTLNNPCWKKLQAPWE                                  :                                                      1m1n(E) : DGFAIFARDMDMTLNNPCWKKLQAPWE                                    ___________________________

Details

Property   Value  Conformer 1 1l5h(A)  Conformer 2 1m1n(E)  Radius gyration for conformer 1 23.14 A  Radius gyration for conformer 2 22.76 A  EC Number 1.18.6.1   Click here to see the DynDom results or the famliy  Trigger ligands HCA8494  Trigger ligands in which conformation Conformation  2  Conformaton with trigger ligands is compact Yes  Spanning ligands Yes  Residues in enzyme contacting ligands ALA65  ARG96  GLN191  GLY424  HIS442  ILE425  LYS426   Residues in extended bending regions contacting ligands GLY424  ILE425  LYS426   Residues in extended bending regions making H-bonds or salt-bridges with ligands ILE425    Residues in extended bending regions with an H-bond between its main chain and ligands ILE425  Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT