3-PHOSPHOGLYCERATE KINASE
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

__________________________________________________   16pk    : EKKSINECDLKGKKVLIRVDFNVPVKNGKITNDYRIRSALPTLKKVLTEG           :                                                      13pk(C) : EKKSINECDLKGKKVLIRVDFNVPVKNGKITNDYRIRSALPTLKKVLTEG             __________________________________________________             __________________________________________________   16pk    : GSCVLMSHLGRPKGIPMAQAGKIRSTGGVPGFQQKATLKPVAKRLSELLL           :                                                      13pk(C) : GSCVLMSHLGRPKGIPMAQAGKIRSTGGVPGFQQKATLKPVAKRLSELLL             __________________________________________________             __________________________________________________   16pk    : RPVTFAPDCLNAADVVSKMSPGDVVLLENVRFYKEEGSKKAKDREAMAKI           :                                                      13pk(C) : RPVTFAPDCLNAADVVSKMSPGDVVLLENVRFYKEEGSKKAKDREAMAKI             __________________________________________________             ____________________________________________201___   16pk    : LASYGDVYISDAFGTAHRDSATMTGIPKILGNGAAGYLMEKEISYFAKVL           :                                                      13pk(C) : LASYGDVYISDAFGTAHRDSATMTGIPKILGNGAAGYLMEKEISYFAKVL             ____________________________________________201___             _________216______________________________________   16pk    : GNPPRPLVAIVGGAKVSDKIQLLDNMLQRIDYLLIGGAMAYTFLKAQGYS           :                                                      13pk(C) : GNPPRPLVAIVGGAKVSDKIQLLDNMLQRIDYLLIGGAMAYTFLKAQGYS             _________216______________________________________             __________________________________________________   16pk    : IGKSKCEESKLEFARSLLKKAEDRKVQVILPIDHVCHTEFKAVDSPLITE           :                                                      13pk(C) : IGKSKCEESKLEFARSLLKKAEDRKVQVILPIDHVCHTEFKAVDSPLITE             __________________________________________________             __________________________________________________   16pk    : DQNIPEGHMALDIGPKTIEKYVQTIGKCKSAIWNGPMGVFEMVPYSKGTF           :                                                      13pk(C) : DQNIPEGHMALDIGPKTIEKYVQTIGKCKSAIWNGPMGVFEMVPYSKGTF             __________________________________________________             __________________________________391_____________   16pk    : AIAKAMGRGTHEHGLMSIIGGGDSASAAELSGEAKRMSHVSTGGGASLEL           :                                                      13pk(C) : AIAKAMGRGTHEHGLMSIIGGGDSASAAELSGEAKRMSHVSTGGGASLEL             __________________________________391_____________             _______________                                      16pk    : LEGKTLPGVTVLDDK                                              :                                                      13pk(C) : LEGKTLPGVTVLDDK                                                _______________

Details

Property   Value  Conformer 1 16pk( )  Conformer 2 13pk(C)  Radius gyration for conformer 1 23.15 A  Radius gyration for conformer 2 21.9 A  EC Number 2.7.2.3   Click here to see the DynDom results or the famliy  Trigger ligands ADP421  Trigger ligands in which conformation Conformation  2  Conformaton with trigger ligands is compact Yes  Spanning ligands Yes  Residues in enzyme contacting ligands ALA218  ALA242  ALA314  ASN338  ASP377  GLU345  GLY217  GLY241  GLY339  GLY342  GLY375  GLY376  LEU315  LYS219  LYS223  PHE294  PRO340  SER378  TYR245  VAL343   Residues in extended bending regions contacting ligands ALA218  GLY217  LYS219  LYS223   Residues in extended bending regions making H-bonds or salt-bridges with ligands ALA218  LYS223    Residues in extended bending regions with an H-bond between its main chain and ligands ALA218  Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT