phosphoglycerate dehydrogenase
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

__________________________________________________   1wwk(A) : MKVLVAAPLHEKAIQVLKDAGLEVIYEEYPDEDRLVELVKDVEAIIVRSK           :                                                      1wwk(B) : MKVLVAAPLHEKAIQVLKDAGLEVIYEEYPDEDRLVELVKDVEAIIVRSK             __________________________________________________             ________________________________________92________   1wwk(A) : PKVTRRVIESAPKLKVIARAGVGLDNIDVEAAKEKGIEVVNAPAASSRSV           :                                                      1wwk(B) : PKVTRRVIESAPKLKVIARAGVGLDNIDVEAAKEKGIEVVNAPAASSRSV             ________________________________________92________             __________________________________________________   1wwk(A) : AELAVGLMFSVARKIAFADRKMREGVWAKKEAMGIELEGKTIGIIGFGRI           :                                                      1wwk(B) : AELAVGLMFSVARKIAFADRKMREGVWAKKEAMGIELEGKTIGIIGFGRI             __________________________________________________             __________________________________________________   1wwk(A) : GYQVAKIANALGMNILLYDPYPNEERAKEVNGKFVDLETLLKESDVVTIH           :                                                      1wwk(B) : GYQVAKIANALGMNILLYDPYPNEERAKEVNGKFVDLETLLKESDVVTIH             __________________________________________________             __________________________________________________   1wwk(A) : VPLVESTYHLINEERLKLMKKTAILINTSRGPVVDTNALVKALKEGWIAG           :                                                      1wwk(B) : VPLVESTYHLINEERLKLMKKTAILINTSRGPVVDTNALVKALKEGWIAG             __________________________________________________             ________________________________285_______________   1wwk(A) : AGLDVFEEEPLPKDHPLTKFDNVVLTPHIGASTVEAQERAGVEVAEKVVK           :                                                      1wwk(B) : AGLDVFEEEPLPKDHPLTKFDNVVLTPHIGASTVEAQERAGVEVAEKVVK             ________________________________285_______________             ____                                                 1wwk(A) : ILKG                                                         :                                                      1wwk(B) : IL**                                                           ____

Details

Property   Value  Conformer 1 1wwk(A)  Conformer 2 1wwk(B)  Radius gyration for conformer 1 22.32 A  Radius gyration for conformer 2 22.3 A  EC Number 1.1.1.95   Click here to see the DynDom results or the famliy  Trigger ligands NAD2001  Trigger ligands in which conformation Conformation  2  Conformaton with trigger ligands is compact Yes  Spanning ligands Yes  Residues in enzyme contacting ligands ALA281  ARG149  ARG230  ASP169  ASP254  GLY146  GLY148  GLY280  HIS200  HIS278  ILE150  PHE147  PRO170  PRO202  SER206  SER229  SER96  THR207  THR228  TYR168  TYR171  VAL100  VAL201  VAL255  VAL72   Residues in extended bending regions contacting ligands SER96   Residues in extended bending regions making H-bonds or salt-bridges with ligands Null   Residues in extended bending regions with an H-bond between its main chain and ligands Null Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT