Phosphate acetyltransferase
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

_____7____________________________________________   1qzt(B) : VTFLEKISERAKKLNKTIALPETEDIRTLQAAAKILERGIADIVLVGNEA           :                                                      2af3(C) : VTFLEKISERAKKLNKTIALPETEDIRTLQAAAKILERGIADIVLVGNEA             _____7____________________________________________             __________________________________________________   1qzt(B) : DIKALAGDLDLSKAKIVDPKTYEKKDEYINAFYELRKHKGITLENAAEIM           :                                                      2af3(C) : DIKALAGDLDLSKAKIVDPKTYEKKDEYINAFYELRKHKGITLENAAEIM             __________________________________________________             ______________________________134_________________   1qzt(B) : SDYVYFAVMMAKLGEVDGVVSGAAHSSSDTLRPAVQIVKTAKGAALASAF           :                                                      2af3(C) : SDYVYFAVMMAKLGEVDGVVSGAAHSSSDTLRPAVQIVKTAKGAALASAF             ______________________________134_________________             __________________________________________________   1qzt(B) : FIISVPDCEYGSDGTFLFADSGMVEMPSVEDVANIAVISAKTFELLVQDV           :                                                      2af3(C) : FIISVPDCEYGSDGTFLFADSGMVEMPSVEDVANIAVISAKTFELLVQDV             __________________________________________________             __________________________________________________   1qzt(B) : PKVAMLSYSTKGSAKSKLTEATIASTKLAQELAPDIAIDGELQVDAAIVP           :                                                      2af3(C) : PKVAMLSYSTKGSAKSKLTEATIASTKLAQELAPDIAIDGELQVDAAIVP             __________________________________________________             ____________________________________________298___   1qzt(B) : KVAASKAPGSPVAGKANVFIFPDLNCGNIAYKIAQRLAKAEAYGPITQGL           :                                                      2af3(C) : KVAASKAPGSPVAGKANVFIFPDLNCGNIAYKIAQRLAKAEAYGPITQGL             ____________________________________________298___             ________________________________                     1qzt(B) : AKPINDLSRGCSDEDIVGAVAITCVQAAAQD*                             :                                                      2af3(C) : AKPINDLSRGCSDEDIVGAVAITCVQAAAQDK                               ________________________________

Details

Property   Value  Conformer 1 1qzt(B)  Conformer 2 2af3(C)  Radius gyration for conformer 1 20.76 A  Radius gyration for conformer 2 21.07 A  EC Number 2.3.1.8   Click here to see the DynDom results or the famliy  Trigger ligands COA501 COA502  Trigger ligands in which conformation Conformation  2  Conformaton with trigger ligands is compact No  Spanning ligands Yes  Residues in enzyme contacting ligands ALA148  ALA150  ALA293  ARG87  ASN279  ASP307  CYS312  GLN137  GLN286  GLU176  GLY173  GLY295  LEU132  LEU147  LEU308  LYS283  LYS90  MET174  PHE152  PRO296  SER128  SER309  THR141  THR298  TYR282  TYR294  VAL136  VAL175   Residues in extended bending regions contacting ligands ALA148  ALA150  GLN137  GLY295  LEU132  LEU147  PRO296  THR141  THR298  VAL136   Residues in extended bending regions making H-bonds or salt-bridges with ligands ALA148  GLY295  THR298    Residues in extended bending regions with an H-bond between its main chain and ligands ALA148  GLY295  Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT