Succinyl-CoA ligase [GDP-forming] beta-chain,
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

_____5_____________20_____________________________   1eud(B) : MVNLQEYQSKKLMSDNGVKVQRFFVADTANEALEAAKRLNAKEIVLKAQI           :                                                      2fp4(B) : *MNLQEYQSKKLMSDNGVKVQRFFVADTANEALEAAKRLNAKEIVLKAQI             _____5_____________20_____________________________             __________________________________________________   1eud(B) : LAGGRGKGVFSSGLKGGVHLTKDPEVVGQLAKQMIGYNLATKQTPKEGVK           :                                                      2fp4(B) : LAGGRGKGVFSSGLKGGVHLTKDPEVVGQLAKQMIGYNLATKQTPKEGVK             __________________________________________________             _____107__________________________________________   1eud(B) : VNKVMVAEALDISRETYLAILMDRSCNGPVLVGSPQGGVDIEEVAASNPE           :                                                      2fp4(B) : VNKVMVAEALDISRETYLAILMDRSCNGPVLVGSPQGGVDIEEVAASNPE             _____107__________________________________________             __________________________________________________   1eud(B) : LIFKEQIDIIEGIKDSQAQRMAENLGFLGPLQNQAADQIKKLYNLFLKID           :                                                      2fp4(B) : LIFKEQIDIIEGIKDSQAQRMAENLGFLGPLQNQAADQIKKLYNLFLKID             __________________________________________________             __________________________________________________   1eud(B) : ATQVEVNPFGETPEGQVVCFDAKINFDDNAEFRQKDIFAMDDKSENEPIE           :                                                      2fp4(B) : ATQVEVNPFGETPEGQVVCFDAKINFDDNAEFRQKDIFAMDDKSENEPIE             __________________________________________________             __________________________________________________   1eud(B) : NEAAKYDLKYIGLDGNIACFVNGAGLAMATCDIIFLNGGKPANFLDLGGG           :                                                      2fp4(B) : NEAAKYDLKYIGLDGNIACFVNGAGLAMATCDIIFLNGGKPANFLDLGGG             __________________________________________________             __________________________________________________   1eud(B) : VKESQVYQAFKLLTADPKVEAILVNIFGGIVN-aIIANGITKA-rELELK           :                                                      2fp4(B) : VKESQVYQAFKLLTADPKVEAILVNIFGGIVNCAIIANGITKACRELELK             __________________________________________________             _____________________________________________        1eud(B) : VPLVVRLEGTNVHEAQNILTNSGLPITSAVDLEDAAKKAVASVTK                :                                                      2fp4(B) : VPLVVRLEGTNVHEAQNILTNSGLPITSAVDLEDAAKKAVASVT*                  _____________________________________________

Details

Property   Value  Conformer 1 1eud(B)  Conformer 2 2fp4(B)  Radius gyration for conformer 1 26.05 A  Radius gyration for conformer 2 25.32 A  EC Number 6.2.1.4   Click here to see the DynDom results or the famliy  Trigger ligands GTP301  Trigger ligands in which conformation Conformation  2  Conformaton with trigger ligands is compact Yes  Spanning ligands Yes  Residues in enzyme contacting ligands ALA108  ARG54  ASP220  GLN20  GLU114  GLY52  GLY53  GLY55  ILE111  LEU109  LEU69  LYS222  PHE219  VAL44  VAL67   Residues in extended bending regions contacting ligands ALA108  GLN20  ILE111  LEU109   Residues in extended bending regions making H-bonds or salt-bridges with ligands GLN20  LEU109    Residues in extended bending regions with an H-bond between its main chain and ligands LEU109  Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT