TRYPTOPHANASE
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space) domain 2 is binding domain(fixed in space)

Sequence


           ____________17__________________________________53 
 2oqx(A) : *KHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSEDVFIDLLTD 
         :                                                    
 2c44(C) : FKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSEDVFIDLLTD 
           ____________17__________________________________53 

           __________________________________________________ 
 2oqx(A) : SGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTH--r 
         :                                                    
 2c44(C) : SGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQGR 
           __________________________________________________ 

           __________________________________________________ 
 2oqx(A) : GAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNV 
         :                                                    
 2c44(C) : GAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNV 
           __________________________________________________ 

           __________________________________________________ 
 2oqx(A) : YIKEAFDTGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQ 
         :                                                    
 2c44(C) : YIKEAFDTGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQ 
           __________________________________________________ 

           __________________________________________________ 
 2oqx(A) : PVSLANLKAMYSIAKKYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQI 
         :                                                    
 2c44(C) : PVSLANLKAMYSIAKKYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQI 
           __________________________________________________ 

           __________________________________________________ 
 2oqx(A) : TRETYKYADMLAMSAKKDAMVPMGGLLCMKDDSFFDVYTECRTL-vVQEG 
         :                                                    
 2c44(C) : TRETYKYADMLAMSAKKDAMVPMGGLLCMKDDSFFDVYTECRTLCVVQEG 
           __________________________________________________ 

           _________________________________339_________351__ 
 2oqx(A) : FPTYGGLEGGAMERLAVGLYDGMNLDWLAYRIAQVQYLVDGLEEIGVV-q 
         :                                                    
 2c44(C) : FPTYGGLEGGAMERLAVGLYDGMNLDWLAYRIAQVQYLVDGLEEIGVVCQ 
           _________________________________339_________351__ 

           ___359____________________________________________ 
 2oqx(A) : QAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIRAVEIGSFLLGR 
         :                                                    
 2c44(C) : QAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIRAVEIGSFLLGR 
           ___359____________________________________________ 

           _______________421___427__________________________ 
 2oqx(A) : DPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIKGLT 
         :                                                    
 2c44(C) : DPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENASNIKGLT 
           _______________421___427__________________________ 

           __________________                                 
 2oqx(A) : FTYEPKVLRHFTAKLKEV                                 
         :                                                    
 2c44(C) : FTYEPKVLRHFTAKLKEV                                 
           __________________                                 

Details

  Property   Value
 Conformer 1 2oqx(A)
 Conformer 2 2c44(C)
 Radius gyration for conformer 1 23 A
 Radius gyration for conformer 2 21.57 A
 EC Number 4.1.99.1  
Click here to see the DynDom results or the famliy
 Trigger ligands K1472
K1473
 Trigger ligands in which conformation Conformation  2
 Conformaton with trigger ligands is compact Yes
 Spanning ligands No
 Residues in enzyme contacting ligands GLN107  GLN301  GLU302  GLY55  PRO275 
 Residues in extended bending regions contacting ligands GLY55 
 Residues in extended bending regions making H-bonds or salt-bridges with ligands GLY55 
  Residues in extended bending regions with an H-bond between its main chain and ligands GLY55 
Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT