Tryptophanyl-tRNA synthetase
Ligand-induced domain movement details

Jmol view of liganded conformation 1

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

___4____9________________________35_______________   1mau(A) : MKTIFSGIQPSGVITIGNYIGALRQFVELQHEYNCYFCIVDQHAITVWQD           :                                                      1maw(B) : MKTIFSGIQPSGVITIGNYIGALRQFVELQHEYNCYFCIVDQHAITVWQD             ___4____9________________________35_______________             _________61__65_______74__________________________   1mau(A) : PHELRQNIRRLAALYLAVGIDPTQATLFIQSEVPAHAQAAWMLQCIVYIG           :                                                      1maw(B) : PHELRQNIRRLAALYLAVGIDPTQATLFIQSEVPAHAQAAWMLQCIVYIG             _________61__65_______74__________________________             __________________________________________________   1mau(A) : ELERMTQFKEKSAGKEAVSAGLLTYPPLMAADILLYNTDIVPVGEDQKQH           :                                                      1maw(B) : ELERMTQFKEKSAGKEAVSAGLLTYPPLMAADILLYNTDIVPVGEDQKQH             __________________________________________________             _____________________174__________________________   1mau(A) : IELTRDLAERFNKRYGELFTIPEARIPKVGARIMSLVDPTKKMSKSDPNP           :                                                      1maw(B) : IELTRDLAERFNKRYGELFTIPEARIPKVGARIMSLVDPTKKMSKSDPNP             _____________________174__________________________             __________________________________________________   1mau(A) : KAYITLLDDAKTIEKKIKSAVTDSEGTIRYDKEAKPGISNLLNIYSTLSG           :                                                      1maw(B) : KAYITLLDDAKTIEKKIKSAVTDSEGTIRYDKEAKPGISNLLNIYSTLSG             __________________________________________________             _____________________________________290__________   1mau(A) : QSIEELERQYEGKGYGVFKADLAQVVIETLRPIQERYHHWMESEELDRVL           :                                                      1maw(B) : QSIEELERQYEGKGYGVFKADLAQVVIETLRPIQERYHHWMESEELDRVL             _____________________________________290__________             ____________________________                         1mau(A) : DEGAEKANRVASEMVRKMEQAMGLGRRR                                 :                                                      1maw(B) : DEGAEKANRVASEMVRKMEQAMGLGR**                                   ____________________________

Details

Property   Value  Conformer 1 1mau(A)  Conformer 2 1maw(B)  Radius gyration for conformer 1 20.82 A  Radius gyration for conformer 2 22.06 A  EC Number 6.1.1.2   Click here to see the DynDom results or the famliy  Trigger ligands LTN390  Trigger ligands in which conformation Conformation  1  Conformaton with trigger ligands is compact Yes  Spanning ligands Yes  Residues in enzyme contacting ligands ASP132  GLN107  GLN147  GLN9  GLY7  HIS43  ILE133  MET129  PHE5  SER6  TYR125  VAL141  VAL40   Residues in extended bending regions contacting ligands GLN9  GLY7  PHE5  SER6   Residues in extended bending regions making H-bonds or salt-bridges with ligands Null   Residues in extended bending regions with an H-bond between its main chain and ligands Null Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT