UDP-glucose 4-epimerase
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

__________________________________________________   2udp(A) : MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVIERLGGK           :                                                      1lrk(A) : MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVIERLGGK             __________________________________________________             __________________________________________________   2udp(A) : HPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNN           :                                                      1lrk(A) : HPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNN             __________________________________________________             __________________________________________________   2udp(A) : VNGTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYG           :                                                      1lrk(A) : VNGTLRLISAMRAANVKNFIFSSSATVYGDNPKIPYVESFPTGTPQSPYG             __________________________________________________             __________________________________________________   2udp(A) : KSKLMVEQILTDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNL           :                                                      1lrk(A) : KSKLMVEQILTDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNL             __________________________________________________             ___________214_____________230____________________   2udp(A) : MPYIAQVAVGRRDSLAIFGNDYPTEDGTGVRDYIHVMDLADGHVVAMEKL           :                                                      1lrk(A) : MPYIAQVAVGRRDSLAIFGNDYPTEDGTGVRDYIHVMDLADGHVVAMEKL             ___________214_____________230____________________             ____________265____272_276_____________________300   2udp(A) : ANKPGVHIYNLGAGVGNSVLDVVNAFSKACGKPVNYHFAPRREGDLPAYW           :                                                      1lrk(A) : ANKPGVHIYNLGAGVGNSVLDVVNAFSKACGKPVNYHFAPRREGDLPACW             ____________265____272_276_____________________300             ______________________________________               2udp(A) : ADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQGYPD                       :                                                      1lrk(A) : ADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQGYPD                         ______________________________________

Details

Property   Value  Conformer 1 2udp(A)  Conformer 2 1lrk(A)  Radius gyration for conformer 1 19.14 A  Radius gyration for conformer 2 19.42 A  EC Number 5.1.3.2   Click here to see the DynDom results or the famliy  Trigger ligands UD1341  Trigger ligands in which conformation Conformation  2  Conformaton with trigger ligands is compact No  Spanning ligands Yes  Residues in enzyme contacting ligands ALA216  ARG231  ARG292  ASN179  ASN198  ASN199  ASP295  GLY229  ILE217  LEU200  LEU215  PHE178  PHE218  THR126  TYR233  VAL269  VAL86   Residues in extended bending regions contacting ligands ALA216  ARG231  GLY229  ILE217  LEU215  PHE218  TYR233  VAL269   Residues in extended bending regions making H-bonds or salt-bridges with ligands ALA216  ARG231  PHE218    Residues in extended bending regions with an H-bond between its main chain and ligands ALA216  PHE218  Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT