UDP-N-ACETYLGLUCOSAMINE PYROPHOSPHORYLASE
Ligand-induced domain movement details

Jmol view of liganded conformation 1

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

___________15_________________34__________________   1fwy(B) : NNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAANELGAAHVH           :                                                      1fxj(A) : NNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAANELGAAHVH             ___________15_________________34__________________             ___________65_________________________________101_   1fwy(B) : LVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML           :                                                      1fxj(A) : LVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML             ___________65_________________________________101_             105_______________________________________________   1fwy(B) : YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGI           :                                                      1fxj(A) : YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGI             105_______________________________________________             __________________________________________________   1fwy(B) : VEHKDATDEQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDII           :                                                      1fxj(A) : VEHKDATDEQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDII             __________________________________________________             ______________________227_________________________   1fwy(B) : ALAYQEGREIVAVHPQRLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGV           :                                                      1fxj(A) : ALAYQEGREIVAVHPQRLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGV             ______________________227_________________________             __________________________________________________   1fwy(B) : MLRDPARFDLRGTLTHGRDVEIDTNVIIEGNVTLGHRVKIGTGCVIKNSV           :                                                      1fxj(A) : MLRDPARFDLRGTLTHGRDVEIDTNVIIEGNVTLGHRVKIGTGCVIKNSV             __________________________________________________             ___________________________                          1fwy(B) : IGDDCEISPYTVVEDANLAAACTI***                                  :                                                      1fxj(A) : IGDDCEISPYTVVEDANLAAACTIGPF                                    ___________________________

Details

Property   Value  Conformer 1 1fwy(B)  Conformer 2 1fxj(A)  Radius gyration for conformer 1 22.21 A  Radius gyration for conformer 2 22.33 A  EC Number 2.7.7.23   Click here to see the DynDom results or the famliy  Trigger ligands UD1502  Trigger ligands in which conformation Conformation  1  Conformaton with trigger ligands is compact Yes  Spanning ligands Yes  Residues in enzyme contacting ligands ALA12  ALA13  ALA85  ASN169  ASP105  GLN76  GLN79  GLU154  GLY104  GLY14  GLY140  GLY81  ILE198  LEU11  LEU80  THR199  THR82  TYR103  TYR139  TYR197   Residues in extended bending regions contacting ligands ALA12  ALA13  ASP105  GLY104  GLY14  TYR103   Residues in extended bending regions making H-bonds or salt-bridges with ligands ALA13  ASP105  GLY14  TYR103    Residues in extended bending regions with an H-bond between its main chain and ligands ALA13  GLY14  Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT