URACIL-DNA GLYCOSYLASE
Ligand-induced domain movement details

Jmol view of liganded conformation 1

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

__________________________________________________   1flz(A) : ANELTWHDVLAEEKQQPHFLNTLQTVASERQSGVTIYPPQKDVFNAFRFT           :                                                      1eui(A) : ****TWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDVFNAFRFT             __________________________________________________             _________________________78__82___________________   1flz(A) : ELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIP           :                                                      1eui(A) : ELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIP             _________________________78__82___________________             ____________116__________________________________1   1flz(A) : GFTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISL           :                                                      1eui(A) : GFTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISL             ____________116__________________________________1             53________________________________________________   1flz(A) : INQHREGVVFLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCN           :                                                      1eui(A) : INQHREGVVFLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCN             53________________________________________________             ____________________________                         1flz(A) : HFVLANQWLEQHGETPIDWMPVLPAESE                                 :                                                      1eui(A) : HFVLANQWLEQRGETPIDWMPVLPA***                                   ____________________________

Details

Property   Value  Conformer 1 1flz(A)  Conformer 2 1eui(A)  Radius gyration for conformer 1 16.6 A  Radius gyration for conformer 2 16.95 A  EC Number 3.2.2.3   Click here to see the DynDom results or the famliy  Trigger ligands URA230  Trigger ligands in which conformation Conformation  1  Conformaton with trigger ligands is compact Yes  Spanning ligands Yes  Residues in enzyme contacting ligands ALA76  ASN123  ASP64  GLN63  HIS187  LEU75  PHE77  PRO65  TYR66   Residues in extended bending regions contacting ligands ALA76  LEU75  PHE77   Residues in extended bending regions making H-bonds or salt-bridges with ligands PHE77    Residues in extended bending regions with an H-bond between its main chain and ligands PHE77  Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT