Tripartite Tricarboxylate Transporter Substrate Binding Protein

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ALA-114   THR-115  9.3 9.0 -6.8 10.1 128.6 131.8 3.7  THR-115   VAL-116  5.9 5.9 -3.1 -15.4 53.2 54.1 41.6  VAL-116   PRO-117  2.5 2.6 8.3 6.6 62.0 61.5 33.7  PRO-117   ASN-118  1.7 1.2 0.7 6.3 76.8 77.1 12.1 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ALA-240   VAL-241  4.6 3.9 7.1 -0.1 46.8 44.4 8.5  VAL-241   THR-242  2.5 2.0 -33.4 0.4 57.1 58.1 63.0  THR-242   PHE-243  2.9 2.7 55.9 -43.9 67.9 61.4 29.2 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees