Bifunctional P-450/nadph-P450 Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 HIS-171   PRO-172  4.8 4.3 -2.5 28.4 88.9 83.7 -25.5
 PRO-172   PHE-173  4.1 3.2 -27.1 0.6 64.1 77.0 30.1
 PHE-173   ILE-174  1.0 0.3 -2.4 11.8 132.4 138.8 16.8
 ILE-174   THR-175  1.2 2.2 -1.8 0.5 127.6 124.7 -7.6
 THR-175   SER-176  2.4 2.0 -8.8 1.7 63.7 68.1 15.9
 SER-176   MET-177  4.9 4.7 -2.4 2.6 83.2 83.7 15.0

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ASN-213   ASP-214  5.3 5.4 23.1 -7.6 66.0 68.2 29.2
 ASP-214   LEU-215  5.0 4.9 -11.2 -20.0 89.9 104.9 -40.5
 LEU-215   VAL-216  1.9 1.9 45.3 -7.8 42.5 53.6 95.3
 VAL-216   ASP-217  1.3 1.5 11.1 -24.9 28.1 32.4 -44.5

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees