Bifunctional P-450/nadph-P450 Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 PHE-165   TYR-166  16.5 16.3 0.4 0.6 78.5 81.7 -0.7
 TYR-166   ARG-167  16.9 16.8 6.3 -12.2 33.2 35.8 8.3
 ARG-167   ASP-168  14.6 14.5 8.2 10.7 39.7 31.2 -18.8
 ASP-168   GLN-169  15.3 13.8 -53.4 32.8 114.6 89.5 -56.3
 GLN-169   PRO-170  11.5 10.4 -31.4 -6.1 38.0 20.6 169.5
 PRO-170   HIS-171  8.8 8.4 12.1 8.8 125.6 119.1 -36.1
 HIS-171   PRO-172  7.4 7.5 -4.9 13.3 115.2 112.5 -11.1
 PRO-172   PHE-173  5.2 5.7 -7.6 -14.0 68.8 70.5 9.8

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 LYS-224   ALA-225  5.3 5.0 0.9 3.3 66.4 59.4 -9.5
 ALA-225   SER-226  8.4 8.2 -26.5 8.8 115.0 119.9 -89.0
 GLN-229   SER-230  6.8 5.5 32.9 -27.9 134.3 132.8 -61.6
 SER-230   ASP-231  6.9 7.7 2.8 57.7 76.5 95.2 -50.6
 ASP-231   ASP-232  8.0 10.5 -80.5 45.2 31.6 37.5 166.5
 ASP-232   LEU-233  8.5 8.3 -23.0 -14.9 72.3 99.0 -1.6
 LEU-233   LEU-234  8.3 7.9 18.5 -0.2 80.3 77.0 6.6
 LEU-234   THR-235  4.5 4.2 -3.3 -0.7 160.7 173.3 -36.9
 THR-235   HIS-236  4.5 4.4 -9.8 16.7 103.1 109.1 31.3
 HIS-236   MET-237  7.8 7.6 -7.0 -13.3 61.1 80.2 4.5
 MET-237   LEU-238  7.4 7.3 11.1 13.0 58.2 57.4 5.8
 LEU-238   ASN-239  5.6 4.9 -1.0 21.1 162.2 159.5 99.6

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees