Phosphoglycerate Kinase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LYS-192   TYR-193  8.6 6.6 6.7 -3.0 131.9 132.1 -15.5  TYR-193   PHE-194  7.2 5.0 -5.6 9.7 115.6 110.5 -12.1  PHE-194   GLY-195  5.9 4.3 20.0 -5.0 47.0 56.0 38.1 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ILE-386   SER-387  4.3 2.3 86.4 139.9 144.7 126.0 383.3  SER-387   HIS-388  4.3 3.8 91.3 -33.9 122.9 130.8 -92.0  HIS-388   VAL-389  1.7 0.9 12.5 -15.2 82.0 88.1 21.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-392   GLY-393  7.5 7.9 6.6 -15.6 105.4 95.3 32.1  GLY-393   GLY-394  9.0 9.5 -7.1 6.2 136.6 141.8 3.6  GLY-394   ALA-395  10.4 10.6 14.5 -5.1 48.6 57.6 16.1 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LEU-400   GLU-401  6.7 8.5 18.4 -13.9 120.0 131.1 41.1  GLU-401   GLY-402  9.7 10.8 33.7 -31.6 130.8 127.5 -19.2  GLY-402   LYS-403  8.7 9.6 -23.9 -28.6 24.2 29.1 131.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees