DNA Topoisomerase II, Alpha Isozyme

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 PRO-47   ASP-48  26.4 25.9 25.4 10.2 48.9 52.2 380.4
 ASP-48   THR-49  25.3 24.8 -37.4 -1.5 155.0 136.7 -455.0
 THR-49   TYR-50  25.4 23.5 33.7 -4.9 92.3 107.6 2.8
 TYR-50   ILE-51  22.8 22.0 -3.1 -14.0 66.4 58.3 155.9
 ILE-51   GLY-52  20.5 20.0 -1.3 6.3 90.7 88.4 31.6
 GLY-52   SER-53  21.8 21.0 -3.7 -10.2 42.0 49.8 191.2
 SER-53   VAL-54  23.9 23.7 13.7 -1.5 162.6 163.2 -175.7
 VAL-54   GLU-55  24.6 24.5 -10.7 10.9 59.0 57.1 31.8
 GLU-55   LEU-56  26.2 26.3 -8.5 11.2 84.5 80.7 15.4
 LEU-56   VAL-57  24.9 25.1 -8.2 7.1 142.5 138.7 -16.8
 VAL-57   THR-58  24.3 24.3 -18.1 8.5 68.2 65.4 59.3

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 PRO-263   VAL-264  9.4 9.3 22.9 -18.7 3.7 13.1 83.1
 VAL-264   LYS-265  8.4 8.6 10.4 -15.9 66.8 62.5 -82.1
 LYS-265   GLY-266  10.3 10.2 12.8 -2.4 9.9 16.6 158.3
 GLY-266   PHE-267  8.9 9.3 -27.0 9.9 102.0 96.5 -23.5
 PHE-267   ARG-268  8.3 8.0 16.0 -9.9 101.9 96.2 -26.9
 ARG-268   SER-269  12.1 11.8 7.1 -5.8 159.9 158.4 -13.7
 SER-269   TYR-270  12.3 12.2 12.2 -8.8 101.2 106.4 -16.0

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 LEU-275   LYS-276  18.2 18.0 -11.9 62.6 42.9 55.4 -600.5
 VAL-288   ILE-289  19.6 19.7 31.5 -21.9 51.3 51.9 85.9

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees