Ubiquitin-Like 2 Activating Enzyme E1b

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PRO-437   ASN-438  1.6 1.7 3.6 7.1 86.7 88.1 2.5  ASN-438   PRO-439  3.8 3.9 2.5 -1.8 135.8 126.3 0.9  PRO-439   ASN-440  4.7 4.8 15.1 -39.7 132.5 141.5 113.0  ASN-440   CYS-441  7.3 6.8 24.7 -7.4 132.4 124.7 -91.7  CYS-441   TYR-442  5.6 5.2 12.8 2.1 160.1 161.9 -94.6  TYR-442   VAL-443  6.1 5.5 -4.5 -6.0 62.3 68.7 53.2  VAL-443   CYS-444  3.9 3.0 -11.9 19.8 110.7 116.2 44.4  CYS-444   ALA-445  1.8 2.0 -12.1 14.9 106.1 106.1 -11.9  ALA-445   SER-446  2.1 1.8 7.7 -15.2 127.8 121.6 32.4  SER-446   LYS-447  4.8 5.0 -8.4 5.5 22.7 20.7 17.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLN-518   ALA-519  6.3 6.1 -14.0 15.9 109.0 106.1 8.8  ALA-519   ASP-520  4.2 4.1 1.4 -3.8 111.7 109.0 14.1  ASP-520   ASP-521  1.1 1.0 13.6 -17.9 78.2 79.1 -0.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  TYR-526   THR-527  2.9 2.8 1.2 -2.1 124.9 122.2 -2.5  THR-527   LEU-528  0.8 1.1 20.5 -19.4 61.0 56.1 6.1  LEU-528   LEU-529  4.2 4.1 -23.0 22.0 59.8 64.4 24.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees