Ribose Abc Transporter, Periplasmic Ribose- Binding Protein

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLN-101   ILE-102  8.7 8.6 17.5 -18.8 126.1 127.3 34.4  ILE-102   TYR-103  6.8 6.5 -14.3 -4.7 81.5 78.8 13.6  TYR-103   SER-104  3.3 3.4 11.3 10.3 117.8 95.1 -39.0  SER-104   ASP-105  0.2 0.8 27.1 -3.9 47.4 34.9 83.3  ASP-105   ASN-106  3.2 3.4 1.0 -7.0 91.0 88.6 20.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  MET-243   GLN-244  6.5 6.6 -11.6 5.2 53.7 54.6 14.5  GLN-244   PHE-245  3.2 3.7 6.2 3.4 48.1 42.7 22.7  PHE-245   PRO-246  0.3 1.1 4.5 1.4 108.3 101.4 6.0  PRO-246   LYS-247  3.8 4.8 -0.1 0.4 117.5 120.1 -4.2  LYS-247   LEU-248  5.6 5.6 -3.8 3.6 69.4 67.2 5.8  LEU-248   MET-249  5.8 5.5 -13.8 7.9 24.0 33.5 -1.2  MET-249   ALA-250  6.0 6.2 -2.7 0.1 92.8 97.6 17.2 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ILE-270   VAL-271  7.4 7.4 8.3 13.4 141.1 137.5 27.9  VAL-271   PRO-272  7.1 6.9 14.1 -5.0 124.3 105.9 -3.6  PRO-272   VAL-273  4.6 4.0 -2.4 -1.0 20.8 16.1 -11.7  VAL-273   THR-274  4.5 3.3 11.1 -8.3 102.8 97.8 15.1  THR-274   VAL-275  5.7 5.0 -13.2 5.0 49.0 56.9 23.2  VAL-275   GLU-276  5.8 5.4 -8.8 7.5 65.6 65.9 34.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees