CYTIDYLATE KINASE
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

__________________________________________________   1kdt(B) : AIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLALAALHHHV           :                                                      2cmk(A) : AIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLALAALHHHV             __________________________________________________             __________________________________________________   1kdt(B) : DVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ           :                                                      2cmk(A) : DVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ             __________________________________________________             ____________________________________________149___   1kdt(B) : VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSE           :                                                      2cmk(A) : VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSE             ____________________________________________149___             _____________________________________192__________   1kdt(B) : ERAHRRMLQLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVL           :                                                      2cmk(A) : ERAHRRMLQLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVL             _____________________________________192__________             _______________________                              1kdt(B) : DSTTLSIEQVIEKALQYARQKLA                                      :                                                      2cmk(A) : DSTTLSIEQVIEKALQYARQK**                                        _______________________

Details

Property   Value  Conformer 1 1kdt(B)  Conformer 2 2cmk(A)  Radius gyration for conformer 1 16.99 A  Radius gyration for conformer 2 17.41 A  EC Number 2.7.4.14   Click here to see the DynDom results or the famliy  Trigger ligands CDP407  Trigger ligands in which conformation Conformation  2  Conformaton with trigger ligands is compact No  Spanning ligands Yes  Residues in enzyme contacting ligands ALA104  ARG110  ARG131  ARG188  ARG41  ASP132  ASP185  GLY130  GLY37  SER101  SER14  SER36  TYR40   Residues in extended bending regions contacting ligands Null  Residues in extended bending regions making H-bonds or salt-bridges with ligands Null   Residues in extended bending regions with an H-bond between its main chain and ligands Null Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT