ASPARTYL-TRNA SYNTHETASE
Ligand-induced domain movement details

Jmol view of liganded conformation 1

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

__________________________________________________   1g51(B) : MRRTHYAGSLRETHVGEEVVLEGWVNRRRDLGGLIFLDLRDREGLVQLVA           :                                                      1efw(A) : MRRTHYAGSLRETHVGEEVVLEGWVNRRRDLGGLIFLDLRDREGLVQLVA             __________________________________________________             __________________________________________________   1g51(B) : HPASPAYATAERVRPEWVVRAKGLVRLRPEPNPRLATGRVEVELSALEVL           :                                                      1efw(A) : HPASPAYATAERVRPEWVVRAKGLVRLRPEPNPRLATGRVEVELSALEVL             __________________________________________________             __________________________________________________   1g51(B) : AEAKTPPFPVDAGWRGEEEKEASEELRLKYRYLDLRRRRMQENLRLRHRV           :                                                      1efw(A) : AEAKTPPFPVDAGWRGEEEKEASEELRLKYRYLDLRRRRMQENLRLRHRV             __________________________________________________             __________________________________________________   1g51(B) : IKAIWDFLDREGFVQVETPFLTKSTPEGARDFLVPYRHEPGLFYALPQSP           :                                                      1efw(A) : IKAIWDFLDREGFVQVETPFLTKSTPEGARDFLVPYRHEPGLFYALPQSP             __________________________________________________             __________________________________________________   1g51(B) : QLFKQMLMVAGLDRYFQIARCFRDEDLRADRQPDFTQLDLEMSFVEVEDV           :                                                      1efw(A) : QLFKQMLMVAGLDRYFQIARCFRDEDLRADRQPDFTQLDLEMSFVEVEDV             __________________________________________________             _____________________________________________1299_   1g51(B) : LELNERLMAHVFREALGVELPLPFPRLSYEEAMERYGSDKPDLRFGLELK           :                                                      1efw(A) : LELNERLMAHVFREALGVELPLPFPRLSYEEAMERYGSDKPDLRFGLELK             ______________________________________________299_             __________________________________________________   1g51(B) : EVGPLFRQSGFRVFQEAESVKALALPKALSRKEVAELEEVAKRHKAQGLA           :                                                      1efw(A) : EVGPLFRQSGFRVFQEAESVKALALPKALSRKEVAELEEVAKRHKAQGLA             __________________________________________________             ________________________________________________14   1g51(B) : WARVEEGGFSGGVAKFLEPVREALLQATEARPGDTLLFVAGPRKVAATAL           :                                                      1efw(A) : WARVEEGGFSGGVAKFLEPVREALLQATEARPGDTLLFVAGPRKVAATAL             _________________________________________________4             02_1407___________________________________________   1g51(B) : GAVRLRAADLLGLKREGFRFLWVVDFPLLEWDEEEEAWTYMHHPFTSPHP           :                                                      1efw(A) : GAVRLRAADLLGLKREGFRFLWVVDFPLLEWDEEEEAWTYMHHPFTSPHP             02__407___________________________________________             __________________________________________________   1g51(B) : EDLPLLEKDPGRVRALAYDLVLNGVEVGGGSIRIHDPRLQARVFRLLGIG           :                                                      1efw(A) : EDLPLLEKDPGRVRALAYDLVLNGVEVGGGSIRIHDPRLQARVFRLLGIG             __________________________________________________             __________________________________________________   1g51(B) : EEEQREKFGFFLEALEYGAPPHGGIAWGLDRLLALMTGSPSIREVIAFPK           :                                                      1efw(A) : EEEQREKFGFFLEALEYGAPPHGGIAWGLDRLLALMTGSPSIREVIAFPK             __________________________________________________             ______________________________                       1g51(B) : NKEGKDPLTGAPSPVPEEQLRELGLMVVRP                               :                                                      1efw(A) : NKEGKDPLTGAPSPVPEEQLRELGLMVVRP                                 ______________________________

Details

Property   Value  Conformer 1 1g51(B)  Conformer 2 1efw(A)  Radius gyration for conformer 1 29.58 A  Radius gyration for conformer 2 29.77 A  EC Number 6.1.1.12   Click here to see the DynDom results or the famliy  Trigger ligands AMP800  Trigger ligands in which conformation Conformation  1  Conformaton with trigger ligands is compact Yes  Spanning ligands No  Residues in enzyme contacting ligands ALA1350  ALA1390  GLN1347  GLU1338  GLY1348  LEU1349  PHE1311  PHE1388  VAL1313  VAL1389   Residues in extended bending regions contacting ligands Null  Residues in extended bending regions making H-bonds or salt-bridges with ligands Null   Residues in extended bending regions with an H-bond between its main chain and ligands Null Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT