ASPARTYL-TRNA SYNTHETASE
Ligand-induced domain movement details

Jmol view of liganded conformation 1

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

__________________________________________________   1c0a(A) : MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGIVQVFFD           :                                                      1il2(A) : MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGIVQVFFD             __________________________________________________             __________________________________________________   1c0a(A) : PDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLT           :                                                      1il2(A) : PDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLT             __________________________________________________             __________________________________________________   1c0a(A) : IINRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRR           :                                                      1il2(A) : IINRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRR             __________________________________________________             __________________________________________________   1c0a(A) : FMDDHGFLDIETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQL           :                                                      1il2(A) : FMDDHGFLDIETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQL             __________________________________________________             __________________________________________________   1c0a(A) : LMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFMTAPQVREVMEA           :                                                      1il2(A) : LMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFMTAPQVREVMEA             __________________________________________________             __________________271____278__283_287_____________   1c0a(A) : LVRHLWLEVKGVDLGDFPVMTFAEAERRYGSDKPDLRNPMELTDVADLLK           :                                                      1il2(A) : LVRHLWLEVKGVDLGDFPVMTFAEAERRYGSDKPDLRNPMELTDVADLLK             __________________271____278__283_287_____________             ____________________323___________________________   1c0a(A) : SVEFAVFAGPANDPKGRVAALRVPGGASLTRKQIDEYGNFVKIYGAKGLA           :                                                      1il2(A) : SVEFAVFAGPANDPKGRVAALRVPGGASLTRKQIDEYGNFVKIYGAKGLA             ____________________323___________________________             __________________________________________________   1c0a(A) : YIKVNERAKGLEGINSPVAKFLNAEIIEDILDRTAAQDGDMIFFGADNKK           :                                                      1il2(A) : YIKVNERAKGLEGINSPVAKFLNAEIIEDILDRTAAQDGDMIFFGADNKK             __________________________________________________             ____________415______424__________________________   1c0a(A) : IVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP           :                                                      1il2(A) : IVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP             ____________415______424__________________________             __________________________________________________   1c0a(A) : FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVF           :                                                      1il2(A) : FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVF             __________________________________________________             _____________________________________540__545_____   1c0a(A) : GILGINEEEQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRD           :                                                      1il2(A) : GILGINEEEQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRD             _____________________________________540__545_____             ___________________________________                  1c0a(A) : VIAFPKTTAAACLMTEAPSFANPTALAELSIQVVK                          :                                                      1il2(A) : VIAFPKTTAAACLMTEAPSFANPTALAELSIQVVK                            ___________________________________

Details

Property   Value  Conformer 1 1c0a(A)  Conformer 2 1il2(A)  Radius gyration for conformer 1 29.84 A  Radius gyration for conformer 2 29.95 A  EC Number 6.1.1.12   Click here to see the DynDom results or the famliy  Trigger ligands AMP800  Trigger ligands in which conformation Conformation  1  Conformaton with trigger ligands is compact Yes  Spanning ligands No  Residues in enzyme contacting ligands ALA350  ALA396  GLY348  LEU349  LYS347  PHE304  PRO367  VAL306  VAL368   Residues in extended bending regions contacting ligands Null  Residues in extended bending regions making H-bonds or salt-bridges with ligands Null   Residues in extended bending regions with an H-bond between its main chain and ligands Null Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT