Erythronate-4-phosphate dehydrogenase
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

__________________________________________________   2o4c(B) : MRILADENIPVVDAFFADQGSIRRLPGRAIDRAALAEVDVLLVRSVTEVS           :                                                      2o4c(A) : MRILADENIPVVDAFFADQGSIRRLPGRAIDRAALAEVDVLLVRSVTEVS             __________________________________________________             ____________________________________88____________   2o4c(B) : RAALAGSPVRFVGTCTIGTDHLDLDYFAEAGIAWSSAPGCNARGVVDYVL           :                                                      2o4c(A) : RAALAGSPVRFVGTCTIGTDHLDLDYFAEAGIAWSSAPGCNARGVVDYVL             ____________________________________88____________             __________________________________________________   2o4c(B) : GCLLAMAEVRGADLAERTYGVVGAGQVGGRLVEVLRGLGWKVLVCDPPRQ           :                                                      2o4c(A) : GCLLAMAEVRGADLAERTYGVVGAGQVGGRLVEVLRGLGWKVLVCDPPRQ             __________________________________________________             __________________________________________________   2o4c(B) : AREPDGEFVSLERLLAEADVISLHTPLNRDGEHPTRHLLDEPRLAALRPG           :                                                      2o4c(A) : AREPDGEFVSLERLLAEADVISLHTPLNRDGEHPTRHLLDEPRLAALRPG             __________________________________________________             __________________________________________________   2o4c(B) : TWLVNASRGAVVDNQALRRLLEGGADLEVALDVWEGEPQADPELAARCLI           :                                                      2o4c(A) : TWLVNASRGAVVDNQALRRLLEGGADLEVALDVWEGEPQADPELAARCLI             __________________________________________________             ________261_____________________285_______________   2o4c(B) : ATPHIAGYSLEGKLRGTAQIYQAYCAWRGIAERVSLQDVLPETWLAGLQL           :                                                      2o4c(A) : ATPHIAGYSLEGKLRGTAQIYQAYCAWRGIAERVSLQDVLPETWLAGLQL             ________261_____________________285_______________             __________________________________________________   2o4c(B) : NPGCDPAWALATLCRAVYDPRSDDAAFRRSLTGDSATRRAAFDALRKHYP           :                                                      2o4c(A) : NPGCDPAWALATLCRAVYDPRSDDAAFRRSLTGDSATRRAAFDALRKHYP             __________________________________________________             ______________________________                       2o4c(B) : PRREITGLRVATGGQAELQRVVRALGAQLV                               :                                                      2o4c(A) : PRREITGLRVATGGQAELQRVVRALGAQLV                                 ______________________________

Details

Property   Value  Conformer 1 2o4c(B)  Conformer 2 2o4c(A)  Radius gyration for conformer 1 21.98 A  Radius gyration for conformer 2 21.7 A  EC Number 1.1.1.290   Click here to see the DynDom results or the famliy  Trigger ligands NAD1002  Trigger ligands in which conformation Conformation  2  Conformaton with trigger ligands is compact Yes  Spanning ligands Yes  Residues in enzyme contacting ligands ALA124  ALA206  ALA256  ARG208  ASN91  ASP146  ASP232  CYS145  GLN126  GLY123  GLY125  GLY257  HIS174  HIS183  HIS254  ILE67  PRO147  PRO148  PRO176  SER207  THR175  THR185  VAL122  VAL127  VAL233  VAL95   Residues in extended bending regions contacting ligands ASN91   Residues in extended bending regions making H-bonds or salt-bridges with ligands Null   Residues in extended bending regions with an H-bond between its main chain and ligands Null Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT