Folylpolyglutamate synthase
Ligand-induced domain movement details

Jmol view of liganded conformation 1

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

__________________________________________________   1jbw(A) : MNYTETVAYIHSFPRLAKTGDHRRILTLLHALGNPQQQGRYIHVTGTNGK           :                                                      2gc5(A) : MNYTETVAYIHSFPRLAK--dHRRILTLLHALGNPQQQGRYIHVTGTNGK             __________________________________________________             ___________________71_____________________________   1jbw(A) : GSAANAIAHVLEASGLTVGLYTSPFIMRFNERIMIDHEPIPDAALVNAVA           :                                                      2gc5(A) : SSAANAIAHVLEASGLTVGLYTSPFIMRFNERIMIDHEPIPDAALVNAVA             ___________________71_____________________________             __________________________________________________   1jbw(A) : FVRAALERLQQQQADFNVTEFEFITALAYWYFRQRQVDVAVIEVGIGGDT           :                                                      2gc5(A) : FVRAALERLQQQQADFNVTEFEFITALGYWYFRQRQVDVAVIEVGIGGDT             __________________________________________________             __________________________________________________   1jbw(A) : DSTNVITPVVSVLTEVALDHQKLLGHTITAIAKHaGIIKRGIPVVTGNLV           :                                                      2gc5(A) : DSTNVITPVVSVLTEVALDHQKLLGHTITAIAKHaGIIKRGIPVVTGNLV             __________________________________________________             __________________________________________________   1jbw(A) : PDAAAVVAAKVATTGSQWLRFDRDFSVPKAKLHGWGQRFTYEDQDGRISD           :                                                      2gc5(A) : PDAAAVVAAKVATTGSQWLRFDRDFSVPKAKLHGWGQRFTYEDQDGRISD             __________________________________________________             _______________________________________293________   1jbw(A) : LEVPLVGDYQQRNMAIAIQTAKVYAKQTEWPLTPQNIRQGLAASHWPARL           :                                                      2gc5(A) : LEVPLVGDYQQRNMAIAIQTAKVYAKQTEWPLTPQNIRQGLAASHWPARL             _______________________________________293________             __________________________________________________   1jbw(A) : EKISDTPLIVIDGAHNPDGINGLITALKQLFSQPITVIAGILADKDYAAM           :                                                      2gc5(A) : EKISDTPLIVIDGAHNPDGINGLITALKQLFSQPITVIAGI-----yAAM             __________________________________________________             __________________________________________________   1jbw(A) : ADRLTAAFSTVYLVPVPGT-----rLKDSWQEALAASLNDVPDQPIVITG           :                                                      2gc5(A) : ADRLTAAFSTVYLVPVPGTPRALPrLKDSWQEALAASLNDVPDQPIVITG             __________________________________________________             ______________                                       1jbw(A) : SLYLASAVRQTLLG                                               :                                                      2gc5(A) : SLYLASAVRQTLLG                                                 ______________

Details

Property   Value  Conformer 1 1jbw(A)  Conformer 2 2gc5(A)  Radius gyration for conformer 1 21.27 A  Radius gyration for conformer 2 21.84 A  EC Number 6.3.2.17   Click here to see the DynDom results or the famliy  Trigger ligands ACQ999  Trigger ligands in which conformation Conformation  1  Conformaton with trigger ligands is compact Yes  Spanning ligands Yes  Residues in enzyme contacting ligands ALA299  ALA315  ARG300  ASN264  ASN48  ASP313  ASP319  GLU143  GLU165  GLY314  GLY320  GLY49  GLY51  HIS316  LEU413  LYS50  PRO74  SER412  SER52  SER73  THR47  TRP297  TYR260  TYR414   Residues in extended bending regions contacting ligands ALA299  ARG300  PRO74  SER73  TRP297   Residues in extended bending regions making H-bonds or salt-bridges with ligands SER73  TRP297    Residues in extended bending regions with an H-bond between its main chain and ligands SER73  Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT