Glyoxylate reductase/hydroxypyruvate reductas
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

__________________________________________________   2h1s(C) : *LkVFVTRRIPAEGRVALARAADCEVEQWDSDEPIPAKELERGVAGAHGL           :                                                      2gcg(B) : LMKVFVTRRIPAEGRVALARAADCEVEQWDSDEPIPAKELERGVAGAHGL             __________________________________________________             ____________________________________________102___   2h1s(C) : LCLLSDHVDKRILDAAGANLKVIST-sVGIDHLALDEIKKRGIRVGYTPD           :                                                      2gcg(B) : LCLLSDHVDKRILDAAGANLKVISTMSVGIDHLALDEIKKRGIRVGYTPD             ____________________________________________102___             __________________________________________________   2h1s(C) : VLTDTTAELAVSLLLTTCRRLPEAIEEVKNGGWTSWKPLWLCGYGLTQST           :                                                      2gcg(B) : VLTDTTAELAVSLLLTTCRRLPEAIEEVKNGGWTSWKPLWLCGYGLTQST             __________________________________________________             __________________________________________________   2h1s(C) : VGIIGLGRIGQAIARRLKPFGVQRFLYTGRQPRPEEAAEFQAEFVSTPEL           :                                                      2gcg(B) : VGIIGLGRIGQAIARRLKPFGVQRFLYTGRQPRPEEAAEFQAEFVSTPEL             __________________________________________________             __________________________________________________   2h1s(C) : AAQSDFIVVACSLTPATEGLCNKDFFQK-kETAVFINISRGDVVNQDDLY           :                                                      2gcg(B) : AAQSDFIVVACSLTPATEGLCNKDFFQKMKETAVFINISRGDVVNQDDLY             __________________________________________________             ______________________________________296_________   2h1s(C) : QALASGKIAAAGLDVTSPEPLPTNHPLLTLKNCVILPHIGSATHRTRNT-           :                                                      2gcg(B) : QALASGKIAAAGLDVTSPEPLPTNHPLLTLKNCVILPHIGSATHRTRNTM             ______________________________________296_________             _______________________                              2h1s(C) : sLLAANNLLAGLRGEP-pSEL**                                      :                                                      2gcg(B) : SLLAANNLLAGLRGEPMPSELKL                                        _______________________

Details

Property   Value  Conformer 1 2h1s(C)  Conformer 2 2gcg(B)  Radius gyration for conformer 1 21.96 A  Radius gyration for conformer 2 21.72 A  EC Number 1.1.1.79   Click here to see the DynDom results or the famliy  Trigger ligands NDP2002  Trigger ligands in which conformation Conformation  2  Conformaton with trigger ligands is compact Yes  Spanning ligands Yes  Residues in enzyme contacting ligands ALA215  ARG163  ARG185  ARG188  ARG245  ASP269  CYS216  GLN186  GLY160  GLY162  GLY184  GLY295  GLY84  HIS293  ILE159  ILE164  ILE243  LEU161  SER217  SER244  SER296  THR111  THR183  THR222  VAL83   Residues in extended bending regions contacting ligands GLY295  HIS293  SER296   Residues in extended bending regions making H-bonds or salt-bridges with ligands GLY295  SER296    Residues in extended bending regions with an H-bond between its main chain and ligands GLY295  SER296  Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT