a2,3-sialyltransferase, a2,6-sialyltransferas
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

__________________________________________________   2c83(A) : *KTITLYLDPASLPALNQL-dFTQNNEDKTHPRIFGLSRFKIPDNIITQY           :                                                      2ex1(A) : MKTITLYLDPASLPALNQLMDFTQNNEDKTHPRIFGLSRFKIPDNIITQY             __________________________________________________             __________________________________________________   2c83(A) : QNIHFVELKDNRPTEALFTILDQYPGNIELNIHLNIAHSVQLIRPILAYR           :                                                      2ex1(A) : QNIHFVELKDNRPTEALFTILDQYPGNIELNIHLNIAHSVQLIRPILAYR             __________________________________________________             __________________________________________________   2c83(A) : FKHLDRVSIQQLNLYDDGS-eYVDLEKEENKDISAEIKQAEKQLSHYLLT           :                                                      2ex1(A) : FAHLDRVSIQQLNLYDDGSMEYVDLEKEENKDISAEIKQAEKQLSHYLLT             __________________________________________________             __________________________________________________   2c83(A) : GKIKFDNPTIARYVWQSAFPVKYHFLSTDYFEKAEFLQPLKEYLAENYQK           :                                                      2ex1(A) : GKIKFDNPTIARYVWQSAFPVKYHFLSTDYFEKAEFLQPLKEYLAENYQK             __________________________________________________             _____________________248__________________________   2c83(A) : -dWTAYQQLTPEQQAFYLTLVGFNDEVKQSLEVQQAKFIFTGTTTWEGNT           :                                                      2ex1(A) : MDWTAYQQLTPEQQAFYLTLVGFNDEVKQSLEVQQAKFIFTGTTTAEGNT             _____________________248__________________________             __________________________________________________   2c83(A) : EVREYYAQQQLNLLNHFTQAEGDLFIGDHYKIYFKGHPRGGEINDIILNN           :                                                      2ex1(A) : DVREYYAQQQLNLLNHFTQAEGDLFIGDHYKIYFKGHPRGGEINDYILNN             __________________________________________________             ____331___________________________________________   2c83(A) : AKNITNIPANISFEVL--tGLLPDKVGGVASSLYFSLPKEKISHIIFTSN           :                                                      2ex1(A) : AKNITNIPANISFEVLMMTGLLPDKVGGVASSLYFSLPKEKISHIIFTSN             ____331___________________________________________             ___________388___________________________            2c83(A) : KQVKSKEDALNNPYVKV-rRLGIIDESQVIFWDSLKELGGG                    :                                                      2ex1(A) : K---------nNPYVKVMRRLGIIDASQVIFWDSLKQL***                      ___________388___________________________

Details

Property   Value  Conformer 1 2c83(A)  Conformer 2 2ex1(A)  Radius gyration for conformer 1 22.9 A  Radius gyration for conformer 2 21.81 A  EC Number 2.4.99.4   2.4.99.6   2.4.99.9   Click here to see the DynDom results or the famliy  Trigger ligands C5P1427  Trigger ligands in which conformation Conformation  2  Conformaton with trigger ligands is compact Yes  Spanning ligands Yes  Residues in enzyme contacting ligands GLU338  GLY266  GLY310  HIS311  ILE335  LEU357  LEU37  LYS309  PHE337  PRO312  SER336  SER355  SER356  SER36  THR265   Residues in extended bending regions contacting ligands GLU338  ILE335  PHE337  SER336   Residues in extended bending regions making H-bonds or salt-bridges with ligands GLU338  PHE337  SER336    Residues in extended bending regions with an H-bond between its main chain and ligands PHE337  Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT