long chain fatty acid-CoA ligase
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

__________________________________________________   1ult(A) : AFPSTMMDEELNLWDFLERAAALFGRKEVVSRLHTGEVHRTTYAEVYQRA           :                                                      1v25(B) : AFPSTMMDEELNLWDFLERAAALFGRKEVVSRLHTGEVHRTTYAEVYQRA             __________________________________________________             __________________________________________________   1ult(A) : RRLMGGLRALGVGVGDRVATLGFNHFRHLEAYFAVPGMGAVLHTANPRLS           :                                                      1v25(B) : RRLMGGLRALGVGVGDRVATLGFNHFRHLEAYFAVPGMGAVLHTANPRLS             __________________________________________________             __________________________________________________   1ult(A) : PKEIAYILNHAEDKVLLFDPNLLPLVEAIRGELKTVQHFVVMDEKAPEGY           :                                                      1v25(B) : PKEIAYILNHAEDKVLLFDPNLLPLVEAIRGELKTVQHFVVMDEKAPEGY             __________________________________________________             __________________________________________________   1ult(A) : LAYEEALGEEADPVRVPERAACGMAYTTGTTGLPKGVVYSHRALVLHSLA           :                                                      1v25(B) : LAYEEALGEEADPVRVPERAACGMAYTTGTTGLPKGVVYSHRALVLHSLA             __________________________________________________             __________________________________________________   1ult(A) : ASLVDGTALSEKDVVLPVVPMFHVNAWCLPYAATLVGAKQVLPGPRLDPA           :                                                      1v25(B) : ASLVDGTALSEKDVVLPVVPMFHVNAWCLPYAATLVGAKQVLPGPRLDPA             __________________________________________________             __________________________________________________   1ult(A) : SLVELFDGEGVTFTAGVPTVWLALADYLESTGHRLKTLRRLVVGGSAAPR           :                                                      1v25(B) : SLVELFDGEGVTFTAGVPTVWLALADYLESTGHRLKTLRRLVVGGSAAPR             __________________________________________________             __________________________________________________   1ult(A) : SLIARFERMGVEVRQGYGLTETSPVVVQNFVKSHLESLSEEEKLTLKAKT           :                                                      1v25(B) : SLIARFERMGVEVRQGYGLTETSPVVVQNFVKSHLESLSEEEKLTLKAKT             __________________________________________________             __________________________________________________   1ult(A) : GLPIPLVRLRVADEEGRPVPKDGKALGEVQLKGPWITGGYYGNEEATRSA           :                                                      1v25(B) : GLPIPLVRLRVADEEGRPVPKDGKALGEVQLKGPWITGGYYGNEEATRSA             __________________________________________________             ____________________430___________________________   1ult(A) : LTPDGFFRTGDIAVWDEEGYVEIKDRLKDLIKSGGEWISSVDLENALMGH           :                                                      1v25(B) : LTPDGFFRTGDIAVWDEEGYVEIKDRLKDLIKSGGEWISSVDLENALMGH             ____________________430___________________________             __________________________________________________   1ult(A) : PKVKEAAVVAIPHPKWQERPLAVVVPRGEKPTPEELNEHLLKAGFAKWQL           :                                                      1v25(B) : -----aAVVAIPHPKWQERPLAV-------------nEHLLKAGFAKWQL             __________________________________________________             __________________________________                   1ult(A) : PDAYVFAEEIPRTSAGKFLKRALREQYKNYYGG*                           :                                                      1v25(B) : PDAYVF---------gKFLKRALREQYKNYYGGA                             __________________________________

Details

Property   Value  Conformer 1 1ult(A)  Conformer 2 1v25(B)  Radius gyration for conformer 1 24.41 A  Radius gyration for conformer 2 22.73 A  EC Number 6.2.1.3   Click here to see the DynDom results or the famliy  Trigger ligands ANP1666  Trigger ligands in which conformation Conformation  2  Conformaton with trigger ligands is compact Yes  Spanning ligands Yes  Residues in enzyme contacting ligands ALA304  ARG433  ASP418  GLN322  GLY302  GLY323  GLY325  HIS230  ILE430  LEU326  LYS435  LYS439  PHE229  SER303  THR327  TRP234  TRP444  TYR324  VAL231   Residues in extended bending regions contacting ligands ARG433  ILE430  LYS435  LYS439   Residues in extended bending regions making H-bonds or salt-bridges with ligands LYS435  LYS439    Residues in extended bending regions with an H-bond between its main chain and ligands Null Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT