PHENOL HYDROXYLASE
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

__________________________________________________   1pn0(A) : TKYSESYCDVLIVGAGPAGLMAARVLSEYVRQKPDLKVRIIDKRSTKVYN           :                                                      1pn0(D) : TKYSESYCDVLIVGAGPAGLMAARVLSEYVRQKPDLKVRIIDKRSTKVYN             __________________________________________________             __________________________________________________   1pn0(A) : GQADGLQCRTLESLKNLGLADKILSEANDMSTIALYNPDENGHIRRTDRI           :                                                      1pn0(D) : GQADGLQCRTLESLKNLGLADKILSEANDMSTIALYNPDENGHIRRTDRI             __________________________________________________             __________________________________________________   1pn0(A) : PDTLPGISRYHQVVLHQGRIERRILDSIAEISDTRIKVERPLIPEKMEID           :                                                      1pn0(D) : PDTLPGISRYHQVVLHQGRIERRILDSIAEISDTRIKVERPLIPEKMEID             __________________________________________________             ___________________172____________________________   1pn0(A) : SSKAEDPEAYPVTMTLRYMSEDESTPLQFGHKTENGLFRSNLQTQEEEDA           :                                                      1pn0(D) : SSKAEDPEAYPVTMTLRYMSEDESTPLQFGHKTENGLFRSNLQTQEEEDA             ___________________172____________________________             _204______________________________________________   1pn0(A) : NYRLPEGKEAGEIETVHCKYVIGCDGGHSWVRRTLGFEMIGEQTDYIWGV           :                                                      1pn0(D) : NYRLPEGKEAGEIETVHCKYVIGCDGGHSWVRRTLGFEMIGEQTDYIWGV             _204______________________________________________             __________________________________________________   1pn0(A) : LDAVPASNFPDIRSRCAIHSAESGSIMIIPRENNLVRFYVQLQA----tK           :                                                      1pn0(D) : LDAVPASNFPDIRSRCAIHSAESGSIMIIPRENNLVRFYVQLQArVDRTK             __________________________________________________             __________________________________________________   1pn0(A) : FTPEVVIANAKKIFHPYTFDVQQLDWFTAYHIGQRVTEKFSKDERVFIAG           :                                                      1pn0(D) : FTPEVVIANAKKIFHPYTFDVQQLDWFTAYHIGQRVTEKFSKDERVFIAG             __________________________________________________             __________________________________________________   1pn0(A) : DACHTHSPKAGQGMNTSMMDTYNLGWKLGLVLTGRAKRDILKTYEEERQP           :                                                      1pn0(D) : DACHTHSPKAGQGMNTSMMDTYNLGWKLGLVLTGRAKRDILKTYEEERQP             __________________________________________________             __________________________________________________   1pn0(A) : FAQALIDFDHQFSRLFSGRPAKDVADEMGVSMDVFKEAFVKGNEFASGTA           :                                                      1pn0(D) : FAQALIDFDHQFSRLFSGRPAKDVADEMGVSMDVFKEAFVKGNEFASGTA             __________________________________________________             __________________________________________________   1pn0(A) : INYDENLVTDKKSSKQELAKNCVVGTRFKSQPVVRHSEGLWMHFGDRLVT           :                                                      1pn0(D) : INYDENLVTDKKSSKQELAKNCVVGTRFKSQPVVRHSEGLWMHFGDRLVT             __________________________________________________             __________________________________________________   1pn0(A) : DGRFRIIVFAGKATDATQMSRIKKFAAYLDSENSVISRYTPKGADRNSRI           :                                                      1pn0(D) : DGRFRIIVFAGKATDATQMSRIKKFAAYLDSENSVISRYTPKGADRNSRI             __________________________________________________             __________________________________________________   1pn0(A) : DVITIHSCHRDDIEMHDFPAPALHPKWQYDFIYADCDSWHHPHPKSYQAW           :                                                      1pn0(D) : DVITIHSCHRDDIEMHDFPAPALHPKWQYDFIYADCDSWHHPHPKSYQAW             __________________________________________________             __________________________________________________   1pn0(A) : GVDETKGAVVVVRPDGYTSLVTDLEGTAEIDRYFSGILVEPKEKSGAQTE           :                                                      1pn0(D) : GVDETKGAVVVVRPDGYTSLVTDLEGTAEIDRYFSGILVEPKEKSGAQTE             __________________________________________________             ______                                               1pn0(A) : ADWTKS                                                       :                                                      1pn0(D) : ADWTKS                                                         ______

Details

Property   Value  Conformer 1 1pn0(A)  Conformer 2 1pn0(D)  Radius gyration for conformer 1 26.46 A  Radius gyration for conformer 2 26.62 A  EC Number 1.14.13.7   Click here to see the DynDom results or the famliy  Trigger ligands FAD6041  Trigger ligands in which conformation Conformation  2  Conformaton with trigger ligands is compact No  Spanning ligands Yes  Residues in enzyme contacting ligands ALA15  ALA18  ALA53  ARG189  ARG287  ARG44  ASN371  ASN50  ASP225  ASP357  ASP42  ASP54  CYS224  GLN368  GLN52  GLY14  GLY16  GLY226  GLY356  GLY367  GLY369  GLY55  ILE143  LEU142  LYS43  MET370  PRO144  PRO17  PRO364  SER373  THR334  TRP230  TYR289  VAL13  VAL250   Residues in extended bending regions contacting ligands Null  Residues in extended bending regions making H-bonds or salt-bridges with ligands Null   Residues in extended bending regions with an H-bond between its main chain and ligands Null Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT