Poly(A) polymerase
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

__________________________________________________   2hhp(A) : *QKVFGITGPVSTVGATAAENKLNDSLIQELKKEGSFETEQETANRVQVL           :                                                      1fa0(A) : SQKVFGITGPVSTVGATAAENKLNDSLIQELKKEGSFETEQETANRVQVL             __________________________________________________             __________________________________________________   2hhp(A) : KILQELAQRFVYEVSKKKNMSDGMARDAGGKIFTYGSYRLGVHGPGSDID           :                                                      1fa0(A) : KILQELAQRFVYEVSKKKNMSDGMARDAGGKIFTYGSYRLGVHGPGSDID             __________________________________________________             __________________________________________________   2hhp(A) : TLVVVPKHVTREDFFTVFDSLLRERKELDEIAPVPDAFVPIIKIKFSGIS           :                                                      1fa0(A) : TLVVVPKHVTREDFFTVFDSLLRERKELDEIAPVPDAFVPIIKIKFSGIS             __________________________________________________             __________________________________________________   2hhp(A) : IDLICARLDQPQVPLSLTLSDKNLLRNLDEKDLRALNGTRVTDEILELVP           :                                                      1fa0(A) : IDLICARLDQPQVPLSLTLSDKNLLRNLDEKDLRALNGTRVTDEILELVP             __________________________________________________             __________________________________________________   2hhp(A) : KPNVFRIALRAIKLWAQRRAVYANIFGFPGGVAWAMLVARICQLYPNACS           :                                                      1fa0(A) : KPNVFRIALRAIKLWAQRRAVYANIFGFPGGVAWAMLVARICQLYPNACS             __________________________________________________             __________________________________________________   2hhp(A) : AVILNRFFIILSEWNWPQPVILKPIEDGPLQVRVWNPKIYAQDRSHRMPV           :                                                      1fa0(A) : AVILNRFFIILSEWNWPQPVILKPIEDGPLQVRVWNPKIYAQDRSHRMPV             __________________________________________________             __________________________________________________   2hhp(A) : ITPAYPSMCATHNITESTKKVILQEFVRGVQITNDIFSNKKSWANLFEKN           :                                                      1fa0(A) : ITPAYPSMCATHNITESTKKVILQEFVRGVQITNDIFSNKKSWANLFEKN             __________________________________________________             __________________________________________________   2hhp(A) : DFFFRYKFYLEITAYTRGSDEQHLKWSGLVESKVRLLVMKLEVLAGIKIA           :                                                      1fa0(A) : DFFFRYKFYLEITAYTRGSDEQHLKWSGLVESKVRLLVMKLEVLAGIKIA             __________________________________________________             _________________422________________________460___   2hhp(A) : HPFTKPFESSYCCPTEDDYEMIQDKY----GSHKTETALNALKpKAYLST           :                                                      1fa0(A) : HPFTKPFESSYCCPTEDDYEMIQDKlKLVTDENKEEESIKD-APKAYLST             _________________422________________________460___             __________________________________________________   2hhp(A) : MYIGLDFN----kEKVDIHIPCTEFVNLCRSFNEDYGDHKVFNLALRFVK           :                                                      1fa0(A) : MYIGLDFNIENKKEKVDIHIPCTEFVNLCRSFNEDYGDHKVFNLALRFVK             __________________________________________________             _________________                                    2hhp(A) : GYDLPDEVFDENEKRPS                                            :                                                      1fa0(A) : GYDLPDEVFD*******                                              _________________

Details

Property   Value  Conformer 1 2hhp(A)  Conformer 2 1fa0(A)  Radius gyration for conformer 1 24.25 A  Radius gyration for conformer 2 24.96 A  EC Number 2.7.7.19   Click here to see the DynDom results or the famliy  Trigger ligands 3AT604 POP817  Trigger ligands in which conformation Conformation  2  Conformaton with trigger ligands is compact No  Spanning ligands No  Residues in enzyme contacting ligands ALA237  ASP100  ASP102  GLY233  GLY88  LEU211  LEU92  LYS215  SER89  SER99  THR194  TYR224  TYR87  VAL234  VAL94   Residues in extended bending regions contacting ligands Null  Residues in extended bending regions making H-bonds or salt-bridges with ligands Null   Residues in extended bending regions with an H-bond between its main chain and ligands Null Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT