S-adenosylmethionine synthetase isoform type-
Ligand-induced domain movement details

Jmol view of liganded conformation 1

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

__________________________________________________   2obv(A) : FQSMGVFMFTSESVGEGHPDKICDQISDAVLDAHLKQDPNAKVACETVCK           :                                                      1qm4(A) : ****GAFMFTSESVGEGHPDKICDQISDAVLDAHLKQDPNAKVACETVCK             __________________________________________________             ___________________________________________107____   2obv(A) : TGMVLLCGEITSMAMVDYQRVVRDTIKHIGYDDSAKGFDFKTCNVLVALE           :                                                      1qm4(A) : TGMVLLCGEITSMAMIDYQRVVRDTIKHIGYDDSAKGFDFKTCNVLVALE             ___________________________________________108____             _____________________________________151__________   2obv(A) : QQSPDIAQCVHLDRNEEDVGAGDQGLMFGYATDETEECMPLTIILAHKLN           :                                                      1qm4(A) : QQSP------------eDVGAGDQGLMFGYATDETEECMPLTIVLAHKLN             _____________________________________152__________             _______________________187_____195________________   2obv(A) : ARMADLRRSGLLPWLRPDSKTQVTVQYMQDNGAVIPVRIHTIVISVQHNE           :                                                      1qm4(A) : TRMADLRRSGVLPWLRPDSKTQVTVQYVQDNGAVIPVRVHTIVISVQHNE             _______________________188_____196________________             ______________________________________252_________   2obv(A) : DITLEEMRRALKEQVIRAVVPAKYLDEDTVYHLQPSGRFVIGGPQGDAGV           :                                                      1qm4(A) : DITLEAMREALKEQVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGV             ______________________________________253_________             __________________282_____________________________   2obv(A) : TGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKAGLC           :                                                      1qm4(A) : TGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKAGLC             __________________283_____________________________             _____________________________________________359__   2obv(A) : RRVLVQVSYAIGVAEPLSISIFTYGTSQKTERELLDVVHKNFDLRPGVIV           :                                                      1qm4(A) : RRVLVQVSYAIGVAEPLSISIFTYGTSKKTERELLEVVNKNFDLRPGVIV             _____________________________________________360__             __366________________385__________                   2obv(A) : RDLDLKKPIYQKTACYGHFGRSEFPWEVPRKLVF                           :                                                      1qm4(A) : RDLDLKKPIYQKTACYGHFGRSEFPWEVPKKLVF                             __367________________386__________

Details

Property   Value  Conformer 1 2obv(A)  Conformer 2 1qm4(A)  Radius gyration for conformer 1 21.15 A  Radius gyration for conformer 2 21.26 A  EC Number 2.5.1.6   Click here to see the DynDom results or the famliy  Trigger ligands SAM501  Trigger ligands in which conformation Conformation  1  Conformaton with trigger ligands is compact Yes  Spanning ligands Yes  Residues in enzyme contacting ligands ARG249  ASP179  ASP258  HIS29  ILE252  LYS181  PHE250  PRO30  SER206  SER247   Residues in extended bending regions contacting ligands ARG249  ASP258  ILE252  PHE250   Residues in extended bending regions making H-bonds or salt-bridges with ligands Null   Residues in extended bending regions with an H-bond between its main chain and ligands Null Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT