shikimate 5-dehydrogenase
Ligand-induced domain movement details

Jmol view of liganded conformation 1

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

__________________________________________________   2d5c(A) : MLRFAVLGHPVAHSLSPAMHAFALESLGLEGSYEAWDTPLEALPGRLKEV           :                                                      1wxd(B) : MLRFAVLGHPVAHSLSPAMHAFALESLGLEGSYEAWDTPLEALPGRLKEV             __________________________________________________             _________________________________________________1   2d5c(A) : RRAFRGVNLTLPLKEAALAHLDWVSPEAQRIGAVNTVLQVEGRLFGFNTD           :                                                      1wxd(B) : RRAFRGVNLTLPLKEAALAHLDWVSPEAQRIGAVNTVLQVEGRLFGFNTD             _________________________________________________1             02________________________________________________   2d5c(A) : APGFLEALKAGGIPLKGPALVLGAGGAGRAVAFALREAGLEVWVWNRTPQ           :                                                      1wxd(B) : APGFLEALKAGGIPLKGPALVLGAGGAGRAVAFALREAGLEVWVWNRTPQ             02________________________________________________             __________________________________________________   2d5c(A) : RALALAEEFGLRAVPLEKAREARLLVNATRVGLEDPSASPLPAELFPEEG           :                                                      1wxd(B) : RALALAEEFGLRAVPLEKAREARLLVNATRVGLEDPSASPLPAELFPEEG             __________________________________________________             ___________________________230____________________   2d5c(A) : AAVDLVYRPLWTRFLREAKAKGLKVQTGLPMLAWQGALAFRLWTGLLPDP           :                                                      1wxd(B) : AAVDLVYRPLWTRFLREAKAKGLKVQTGLPMLAWQGALAFRLWTGLLPDP             ___________________________230____________________             _____________                                        2d5c(A) : SGMEEAARRAL**                                                :                                                      1wxd(B) : SGMEEAARRALGV                                                  _____________

Details

Property   Value  Conformer 1 2d5c(A)  Conformer 2 1wxd(B)  Radius gyration for conformer 1 18.11 A  Radius gyration for conformer 2 18.51 A  EC Number 1.1.1.25   Click here to see the DynDom results or the famliy  Trigger ligands SKM301  Trigger ligands in which conformation Conformation  1  Conformaton with trigger ligands is compact Yes  Spanning ligands Yes  Residues in enzyme contacting ligands ASN58  ASN85  ASP100  GLN235  LEU59  LYS64  SER14  SER16  THR60  TYR207  VAL6   Residues in extended bending regions contacting ligands ASP100   Residues in extended bending regions making H-bonds or salt-bridges with ligands ASP100    Residues in extended bending regions with an H-bond between its main chain and ligands Null Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT