tRNA (Guanine-N(1)-)-methyltransferase
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

__________________________________________________   1uaj(A) : GLVPRGSHMWIGVISLFPEMFKAITEFGVTGRAVKHNLLKVECWNPRDFT           :                                                      1ual(A) : ******SHMWIGVISLFPEMFKAITEFGVTGRAVKHNLLKVECWNPRDFT             __________________________________________________             __________________________________________________   1uaj(A) : FDKHKTVDDRPYGGGPGMLMMVQPLRDAIHTAKAAAGEGAKVIYLSPQGR           :                                                      1ual(A) : FDKHKTVDDRPYGGGPGMLMMVQPLRDAIHTAKAAAGEGAKVIYLSPQGR             __________________________________________________             __________________________________________________   1uaj(A) : KLDQGGVTELAQNQKLILVCGRYEGIDERLIQTEIDEEWSIGDYVLTGGE           :                                                      1ual(A) : KLDQGGVTELAQNQKLILVCGRYEGIDERLIQTEIDEEWSIGDYVLTGGE             __________________________________________________             _____________________175__________________________   1uaj(A) : LPAMTLIDAVARFIPGVL----gLLDCPHYTRPEVLEGLTVPPVLMSGHH           :                                                      1ual(A) : LPAMTLIDAVARFIPGVLsFADGLLDCPHYTRPEVLEGLTVPPVLMSGHH             _____________________175__________________________             _________________________________________________    1uaj(A) : EEIRKWRLKQSLQRTWLRRPELLEGLALTDEQRKLLKEAQAEHNS****            :                                                      1ual(A) : EEIRKWRLKQSLQRTWLRRPELLEGLALTDEQRKLLKEAQAEHNSLEHH              _________________________________________________

Details

Property   Value  Conformer 1 1uaj(A)  Conformer 2 1ual(A)  Radius gyration for conformer 1 23.27 A  Radius gyration for conformer 2 23.37 A  EC Number 2.1.1.31   Click here to see the DynDom results or the famliy  Trigger ligands SAH301  Trigger ligands in which conformation Conformation  2  Conformaton with trigger ligands is compact No  Spanning ligands No  Residues in enzyme contacting ligands GLN90  GLU116  GLY113  GLY134  GLY140  GLY141  ILE133  LEU138  LEU87  PRO144  PRO89  SER132  SER88  THR139  TRP131  TYR115  TYR136  TYR86  VAL137   Residues in extended bending regions contacting ligands Null  Residues in extended bending regions making H-bonds or salt-bridges with ligands Null   Residues in extended bending regions with an H-bond between its main chain and ligands Null Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT