CCA-adding enzyme
Ligand-induced domain movement details

Jmol view of liganded conformation 1

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

_________11__15__19_______________________________   1tfy(D) : MKVEEILEKALELVIPDEEEVRKGREAEEELRRRLDELGVEYVFVGSYAR           :                                                      1tfw(A) : MKVEEILEKALELVIPDEEEVRKGREAEEELRRRLDELGVEYVFVGSYAR             _________11__15__19_______________________________             __________________________________________________   1tfy(D) : NTWLKGSLEIDVFLLFPEEFSKEELRERGLEIGKAVLDSYEIRYAEHPYV           :                                                      1tfw(A) : NTWLKGSLEIDVFLLFPEEFSKEELRERGLEIGKAVLDSYEIRYAEHPYV             __________________________________________________             __________________121_________133_________________   1tfy(D) : HGVVKGVEVDVVPCYKLKEPKNIKSAVDRTPFHHKWLEGRIKGKENEVRL           :                                                      1tfw(A) : HGVVKGVEVDVVPCYKLKEPKNIKSAVDRTPFHHKWLEGRIKGKENEVRL             __________________121_________133_________________             __________________________________________________   1tfy(D) : LKGFLKANGIYGAEYKVRGFSGYLCELLIVFYGSFLETVKNARRWTRRTV           :                                                      1tfw(A) : LKGFLKANGIYGAEYKVRGFSGYLCELLIVFYGSFLETVKNARRWTRRTV             __________________________________________________             __________________________________________________   1tfy(D) : IDVAKGEVRKGEEFFVVDPVDEKRNVAANLSLDNLARFVHLCREFMEAPS           :                                                      1tfw(A) : IDVAKGEVRKGEEFFVVDPVDEKRNVAANLSLDNLARFVHLCREFMEAPS             __________________________________________________             __________________________________________________   1tfy(D) : LGFFKPKHPLEIEPERLRKIVEERGTAVFAVKFRKPDIVDDNLYPQLERA           :                                                      1tfw(A) : LGFFKPKHPLEIEPERLRKIVEERGTAVFAVKFRKPDIVDDNLYPQLERA             __________________________________________________             __________________________________________________   1tfy(D) : SRKIFEFLERENFMPLRSAFKASEEFCYLLFECQIKEISRVFRRMGPQFE           :                                                      1tfw(A) : SRKIFEFLERENFMPLRSAFKASEEFCYLLFECQIKEISRVFRRMGPQFE             __________________________________________________             __________________________________________________   1tfy(D) : DERNVKKFLSRNRAFRPFIENGRWWAFEMRKFTTPEEGVRSYASTHWHTL           :                                                      1tfw(A) : DERNVKKFLSRNRAFRPFIENGRWWAFEMRKFTTPEEGVRSYASTHWHTL             __________________________________________________             _____________________________________                1tfy(D) : GKNVGESIREYFEIISGEKLFKEPVTAELCEMMGVKD                        :                                                      1tfw(A) : GKNVGESIREYFEIISGEKLFKEPVTAELCEMMGVKD                          _____________________________________

Details

Property   Value  Conformer 1 1tfy(D)  Conformer 2 1tfw(A)  Radius gyration for conformer 1 27.59 A  Radius gyration for conformer 2 28.52 A  EC Number 2.7.7.25   Click here to see the DynDom results or the famliy  Trigger ligands CTP504  Trigger ligands in which conformation Conformation  1  Conformaton with trigger ligands is compact Yes  Spanning ligands Yes  Residues in enzyme contacting ligands ALA163  ARG224  ARG50  ASP61  GLU59  GLY46  HIS133  LYS152  SER171  SER47  THR130  TYR161  TYR173   Residues in extended bending regions contacting ligands HIS133  THR130   Residues in extended bending regions making H-bonds or salt-bridges with ligands HIS133    Residues in extended bending regions with an H-bond between its main chain and ligands Null Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT